Two endomycorrhiza fungi Glomus mosseae and Glomus intraradices and ectomycorrhiza Pisolithus tinctorius were used to inoculated the seedlings of Cyclobalanopsis glauca, C.glauca is a common species for forestation in karst rocky desertification area in southwest of China. The effects of mycorrhiza on the growth and drought tolerance of the seedlings were studied under three simulated drought conditions in green house pot experiment. Soil water content were maintained 75%-85%, 55%-65% and 35%-45% field capacity respectively. The results shows that, under soil drought condition, the biomass of inoculated treatment was significantly higher than the non-inoculated treatment. Mycorrhizal dependency increase with the soil moisture content reduces. Chlorophyll content of infected treatments were not affected by drought stress except Pisolithus tinctorius treatment, while contrast treatment was significantly decreased under drought condition. Under moderate drought condition, content of soluble sugar in shoots of inoculated treament were significantly higher than the contrast treatment, but the content of proline of Glomus intraradices and Pisolithus tinctorius treatment were significantly lower than that of contrast. The enzymatic activity of super oxide dismutase(SOD), peroxidase(POD) and catalase(CAT) of inoculated seedlings are significantly higher than that of non-mycorrhizal seedlings when the soil water content maitained 55%-65% field capacity. When the soil moisture content maitained 35%-45%, POD activity of all inoculated seedlings are significantly higher than that of contrast, SOD activity of Glomus mosseae and Glomus intraradices treatments are significantly higher than the contrast, while CAT activity of Glomus mosseae and Glomus intraradices treatments are significantly higher than that of non-mycorrhizal treatment.Furthermore, total phosphorus and total potassium content of mycorrhizal seedings significantly higher than that of no inoculated plants under soil drought condition. This research suggest that endomycorrhiza and ectomycorrhiza can infect Cyclobalanopsis glauca seedling. Under drought condition, both mycorrhizal fungi can improve the drought tolerance of the seedling through increasing the biomass, activity of antioxidant enzyme, soluble sugar content and advance the assimilate of nutrient of Cyclobalanopsis glauca seedling. Therefore, mycorrhizal Cyclobalanopsis glauca seedling more easier live under the drought karst environment.