In order to evaluate the regional resource-environmental pressure and to provide a reference for the construction of an ecological civilization, the author proposed an evaluation system considering the Footprint Family (Ecological, Carbon and Water Footprints). This system allowed for the study of resource-environmental pressure in Yunnan Province, China. The results revealed that, in Yunnan Province, the ecological pressure level increased from an above-average grade (Ⅱ_b) to a high grade (Ⅲ_a) in the period between 1990 and 2013. Forest carbon sequestration increased 73.28% in the past 23 years, which neutralized 14.67% of greenhouse gas (GHG) emissions in 2013. The Carbon Footprint increased 325.98%, which led to an increase in the level of GHG emissions from a low grade (Ⅰ_b) to a below-average grade (Ⅱ_a). The Water Footprint per capita grew slightly, and the available water resources were relatively high. As a result, the water resource pressure was very low (Ⅰ_a). However, the available water resources per capita decreased at an annual average rate of 3.66%. Since 1990, resource-environmental pressure in Yunnan Province increased a sub-grade, from a very low grade (Ⅰ_a) to a low grade (Ⅰ_c). Ecological pressure of individual cities varied throughout the province. Kunming, Qujing, Zhaotong, Wenshan, Yuxi, and Honghe each had a very high grade (Ⅲ_b). Baoshan and Chuxiong each had a high grade (Ⅲ_a), while both Dali and Lincang had an above-average grade (Ⅱ_b). Lijiang, Dehong, Xishuangbanna, and Pu'er each had a below-average grade (Ⅱ_a). Nujiang had a low grade (Ⅰ_b), and Diqing had a very low grade (Ⅰ_a). GHG emissions varied spatially as well. Qujing and Kunming both had very high grades (Ⅲ_b) and Yuxi had a high grade (Ⅲ_a). Honghe had an above-average grade (Ⅱ_b) while Xishuangbanna, Nujiang, and Diqing each had a carbon sequestration grade (Ⅰ_s). Finally, Lijiang and Pu'er each had a very low grade (Ⅰ_a), of which was lower than the target set for the control of global climate warming. The rest regions had a low grade (Ⅰ_b). Additionally, water resource pressure varied amongst the cities. Kunming had a very high grade (Ⅲ_b), while Chuxiong had an above-average grade (Ⅱ_b). Yuxi, Dali, and Qujing each had below-average grades (Ⅱ_a), and various other regions had very low grades (Ⅰ_a). The resource-environmental pressure too, was very different amongst the cities of the Yunnan Province. Kunming had a very high grade (Ⅲ_b) while Qujing and Yuxi both had high grades (Ⅲ_a). Honghe had an above-average grade (Ⅱ_b), and Zhaotong, Wenshan, Chuxiong, and Dali each had below-average grades (Ⅱ_a). Finally, Baoshan, Lincang, and Dehong all had low grades (Ⅰ_b), and various other regions had very low grades (Ⅰ_a). In the future, Yunnan Province should firmly protect cultivated land resources, promote agricultural high-new technology, and improve the productivity of land. Additionally, the province should adjust energy consumption structure, develop clean energy, reduce GHG emissions, strengthen forest conservation, and enhance carbon sequestration capacity. These measures would allow for the beautiful Yunnan Province to become a banner for ecological civilization construction in China.