Although plant diversity and soil nutrients content can be affected by plateau pika disturbance, the relationships between plant diversity and soil nutrients of alpine meadows in response to plateau pika disturbance are still unclear. RDA redundancy analysis was used to determine the effect of plateau pika disturbance on the relationship between plant diversity and soil nutrients of alpine meadow by using the active burrow entrance density of plateau pika to substitute for the disturbance levels of plateau pika, in which the disturbance consisted of four levels with density of T₁ (7 active burrows/625 m²), T₂ (12 active burrows/625 m²), T₃ (22 active burrows/625 m²) and T₄ (38 active burrows/625 m²). This study showed that the important value of Kobresia pygmaea, dominant plant, firstly increased and then decreased with the increase of plateau pika disturbance level, while the important value of Anemone rivularis var. flore-minors and Potentilla fragarioides, main associate plants, firstly decreased and then increased with the increase of plateau pika disturbance level. The plant diversity index increased when the plateau pika disturbance increased from T₁ level to T₂ level, and showed a decreasing trend when the plateau pika disturbance exceeded T₂ level. Soil total nitrogen and nitrate nitrogen showed a decreasing trend, and ammonium nitrogen firstly decreased and then increased as the plateau pika disturbance increased, while soil organic carbon and total phosphorus firstly increased and then decreased in the increasing process of the plateau pika disturbance. RDA analysis results showed that the correlation relationships between plant diversity index and nitrate nitrogen at 0-10cm soil depth and potassium at 10-20cm soil depth was significantly positive when plateau pika disturbance ranged from T₁ level to T₃ level, and it became negative when plateau pika disturbance was T₄ level, while the correlation relationships between plant diversity index and total nitrogen at 0-10cm soil depth was significantly negative when plateau pika disturbance increased from T₁ level to T₃ level, and it became positive when plateau pika disturbance reached to T₄ level. The relationship between plant diversity index and ammonium nitrogen at 0-10cm soil depth was negative correlation at T₁ level environment and it became positive at T₂, T₃, T₄ levels. These results indicated that the plateau pika disturbance changed the relationships between plant diversity index and soil nutrients of alpine meadow, and the sensitive disturbance of plateau pika on relationships between plant diversity index and soil nutrients was T₂ level and T₃ level.