Wudalianchi Lake is located in the hilly regions of Songnen Plain in northern Heilongjiang Province. The latitude and longitude of Wudalianchi Lake range from 48.56°N to 48.63°N and 126°E to 126.35°E. From 1719 to 1721, volcanic eruptions occurred in Laohei Mountain and Huoshao Mountain. Basalt flows blocked the Baihe River, a tributary of the Namoer River, to form the bead-like Wudalianchi Lake consisting of five parts (P1-P5). Wudalianchi Lake is the second largest volcanic-dammed lake in China and is also a world geological park that is used for fisheries and tourism. The primary stocking fish are Hypophthalmichthys molitrix and Aristichthys nobilis in part 2 (P2) and part 3 (P3) of Wudalianchi Lake. The lake has a continental monsoon climate, with a long, cold winter and a short, cool summer. The lake freezes in late October and thaws in early May. In July, the temperature rises to 21.0℃, on average. The annual average rainfall is 476.3 mm, mostly accumulating from June to August. The main water sources are rainfall and underground springs, and water flows from P5, P4, P3, and P2 to P1. P3 has the largest water area of 7.58 km², followed by P5 (6.1 km²), P2 (2.63 km²), P4 (0.47 km²), and P1 (0.25 km²). P3 is the deepest region in the lake (average depth, 4.5 m). The average depths are 3.5 m in P2 and P5, and 2.5 m in P1 and P4. Wudalianchi Lake is a shallow eutrophic lake. The species composition, dominant species, density, biomass, and diversity of zooplankton were determined from samples collected from 13 stations in from 2011 to 2013. Based on the survey data, the spatial and temporal distribution of the zooplankton community structure were analyzed, and a reasonable stock density of A. nobilis, one of the key stocking fish in the lake, was discussed. The results showed that 82 species (including unidentified species) belonging to Protozoa, Rotifera, Copepoda and Cladocera were found in Wudalianchi Lake. The number and percentage of protozoan species (including unidentified species) (38, 46.3%) and Rotifera (33, 40.2%) were higher than those of Copepoda (6, 7.3%) and Cladocera (5, 6.2%). The dominant species of zooplankton were Protozoa and Rotifera in Wudalianchi Lake. The dominant species were Tintinnidium entzii, Trichocerca elongata, and so on. The average density and biomass were 3794.98 individuals (ind)/L and 2.4558mg/L respectively. The density of Protozoa was 2853.43 ind/L, and was highest in zooplankton. The biomass of Rotifera was 1.1133mg/L, and was highest in zooplankton. The average values for the Shannon-Wiener index (H'), Pielou index (J), and Margalef index (d) were 2.15, 0.7, and 0.98, respectively. The zooplankton community structure had significant spatial-temporal variation. The spatial distribution of zooplankton density, biomass, H', and J in P5 were higher than in P2 and P3. The spatial and temporal distributions of density, biomass, and diversity indices were similar in P2 and P3. In addition, there were higher values of zooplankton density, biomass, and diversity indices in P2 and P3 in the summer than in the spring and autumn. Based on the survey data of zooplankton biomass, the reasonable stocking amount of A. nobilis was 5.71×10⁵. The present study shed light on the sustainable utilization of fisheries resources in Wudalianchi Lake.