China first country to produce tea,and its artificial cultivation can traced back to 1000 BC. Tea(Camellia sinensis), one of the main economic crops in China, can be grown in the tropical and subtropical provinces. The fibrous roots are the most active parts of the root system and play important roles in absorbing water and nutrients. Although many factors(e.g., climatic and topographic conditions, soil properties) significantly influence the growth, turnover and the storage of nutrients in the fibrous roots, very few studies have investigated the effects of nitrogen fertilization. In this study, the dynamic changes to biomass and nutrient storage in the fibrous roots of tea plants were investigated in an experimental plantation that previously received different rates of nitrogen fertilizer[0, 285 and 712 kg hm^-2 a^-1] for 5 consecutive years to elucidate the effect of N fertilization on growth. The fibrous root samples were collected in the summer and autumn seasons, namely from July 2011 to January 2012, using a soil core. The biomass production of the fibrous roots varied from 0.34 g/dm³to 0.72 g/dm³and the carbon(C), nitrogen(N), phosphorus(P), potassium(K), and magnesium(Mg) storage values were 12.6-25.2 mg/dm³, 4.55-11.2 mg/dm³, 0.47-1.19 mg/dm³, 1.31-4.05 mg/dm³, and 0.30-1.19 mg/dm³, respectively. Biomass and nutrient storage in the fibrous roots showed bimodal monthly variations, being the highest in August, 2011 and January, 2012, but relatively low in July and November 2011. Fibrous root growth was initially promoted by N fertilization, but was inhibited during later stages, which might be explained by an alternative above-ground growth strategy. Nitrogen fertilization significantly affected the biomass production of fibrous roots, but its effects varied depending on the month. Biomass production showed an increasing trend in the treatment without N application in July, August, and October. However, in September the biomass production was relatively higher in the treatment receiving medium N, and rose with increasing N applications in December. The N application rates did not significantly affect the total C concentration and contents in fibrous roots. The N concentrations in the fibrous roots were considerably higher in July, August, and January but lower in September, October, and November,. The C/N ratio was significantly affected by the level of nitrogen fertilizer. The root C:N ratio was higher in the treatment without N fertilizer than in the other treatments in July, and in the treatments with N fertilizers in September, October, and November, However, it was lowest in the treatments with high N fertilizer applications in December and January. Surprisingly, N storage in the fibrous roots was un-affected by N fertilization rates. Nitrogen fertilization led to decreasing concentrations and storage of P, K, and Mg in fibrous roots, but the the decreasing tendency was alleviated by a moderate application of N. In conclusion, N fertilization has significant, but temporally variable effects, on the biomass and nutrient storage in the fibrous roots of C. sinensis.