Abstract:Nitrogen (N) deposition has noticeably increased in China in recent years, which is expected to have influenced the physiological characteristics of Pinus tabulaeformis Carr. To improve our understanding about the effects of elevating N deposition on P. tabulaeformis, the short-term responses of the photosynthetic pigments and nonstructural carbohydrates (NSC) in seedlings with three provenances were studied. We set up a simulated N experiment with three provenances of 3-year-old P. tabulaeformis seedlings on Lingkong Mountain in Shanxi Province. The three provenances were Neimeng (NM), Beijing (BJ), and Shanxi (SX). Five N treatments with three replications were designed, including CK (0 kg hm-2 a-1), N1 (15 kg hm-2 a-1), N2 (25 kg hm-2 a-1), N3(50 kg hm-2 a-1) and N4 (150 kg hm-2 a-1). The source of N was NH4NO3 and fertilization was carried out five times in 2012. The contents of the photosynthetic pigment and nonstructural carbohydrate in the seedlings with three provenances were measured under different N treatments in June, August, and October, 2012. The results showed that: in different growing seasons, photosynthetic pigments exhibited various responses to elevated N deposition in P. tabulaeformis seedlings, regardless of provenance. In the early growing season, the photosynthetic pigment contents showed no significant response to the addition of N in any of the seedlings. In the intermediate growing season, the chlorophyll content of BJ provenance seedlings increased significantly under elevated N deposition treatments, reaching a maximum under the N4 treatment. There was no significant increase in the chlorophyll content of provenances NM and BJ increased after adding N. In the late growing season, the photosynthetic pigment content of provenance SX was lowest under the N1 treatment and increased under the N2, N3, and N4 treatments, with no significant differences to CK. The photosynthetic pigment contents of NM and BJ provenances seedlings were not affected by N deposition. Elevated N deposition promoted the transfer and consumption of nonstructural carbohydrate (NSC) in all three seedlings. In the early growing season, N addition significantly reduced soluble sugar (SS) and total nonstructural carbohydrate (TNC) content in the NM provenance seedlings, the TNC content in BJ provenance seedlings, and the SS content in SX provenance seedlings. In the intermediate growing season, the SS, starch (ST), and TNC contents in NM provenance seedlings were lowest under the N4 treatment. In contrast, in SX provenance seedlings, these three indexes reached minimum values under the N3, N1, and N2 treatments, respectively. The SS, ST, and TNC in BJ provenance seedlings showed no significant responses to N deposition. In the late growing season, SS and TNC content noticeably increased in the seedling needles of all three provenances; however, they exhibited various responses to different N deposition levels. The TNC contents in the seedlings of NM and BJ provenances were not significantly influenced by elevated N deposition. High N deposition (N4 treatment) inhibited SS accumulation in NM provenance seedlings, but increased SS content in BJ provenance seedlings. In addition, high N deposition clearly inhibited ST in BJ provenance seedlings, but increased ST content in NM provenance seedlings. Elevated N deposition significantly inhibited NSC accumulation in SX provenance seedlings, which delayed seedling dormancy and extended the growing period of seedlings.