The Orius sauteri (Poppius) is an important predatory natural enemy with a broad diet menu. As an independent biological control agent, O. sauteri was imported to the major regions of agroforestry ecosystem in China. There is no good artificial rearing technology for, which makes it difficult to be used as biocontrol agent in a large scale now. So it is very important to protection and utilization of natural population. In the present paper, the phototaxis behavior of Orius sauteri (Poppius) in response to spectral sensitivity was investigated at 14 monochromatic lights selected through 340nm to 605nm. The results were as follows: (1) Each monochromatic light can cause Orius sauteri phototaxis behavioral responses, phototaxis behavior reponse curves of female and male are multimodal, the primary area in ultraviolet (340 nm, 360 nm and 380 nm), the secondary in green (524 nm), and the rest in orange red light district (583 nm, 605 nm ). (2) Each monochromatic light can cause Orius sauteri (Poppius) photophobism behavioral responses, the curve of female is multimodal, the primary peak at green (400 nm), the male's curve is undulation, the primary peak at blue (483 nm). (3) The effect of sex to response to the spectral sensitivity is not significant, but the response rate values is significant. The phototaxis highest response rate of female was 47.50% at 380 nm,the male was 35.00% at 360 nm. The photophobism highest response rate of female was 19.17% at 400 nm, and the male was 11.25% at 483 nm. It can be concluded that the spectrum of light stimulus gave rise to the phototaxis of Orius sauteri (Poppius) and have significant between the wavelength, gender is an important factor affecting the spectrum of behavioral responses.