Enhanced ozone concentration and depressed solar irradiation were simulated by using open-top chambers (OTCs) method and shading net techniques at 100 nL/L ozone fumigation assembling with three different levels of depressed solar irradiance. The effect of enhanced O₃ combination with depressed solar irradiance was also studied. Two experiments were performed over two years. The combination of three OTCs with six shaded chambers was set up. Each OTC fed with 100 nL/L ozone. In the shaded chambers, solar irradiance was reduced to different levels of 20%, 40%, 60% respectively. Combined actions of O₃ and depressed solar irradiance were tested by shading net above OTCs. Using unshaded OTC and unfed with O₃ as controls. OTC fed with 100 nL/L ozone only, and shaded chamber solar irradiance reduced 20% only were applied too. Each chamber was equivalent and repeated three times. Winter wheat was grown to all life stage in OTCs, shading net and their combine chambers. From reviving to mature stages, in OTCs the plants were treated for 8 h (8:00-16:00) every sunny day with O₃, in shading chambers covered net all life stage. The ability of winter wheat (Triticumaestivum)rhizosphere microbial to use different carbon sources were measured by applying BIOLOG ECO MicroPlate and Average WelL-Color Development (AWCD). Microbial diversity index and Principal Component Analysis (PCA) were calculated on the utilization of different carbon sources. Each treatment was assayed in triplicate. The data were initially compared by analysis of variance and differences between means were detected using the Duncans Multiple Range Tests. Values of P < 0.05 indicated significance. Quite similar results were obtained during the two years' experiments. The results suggesting that under ozone fumigation and reduced solar irradiance, AWCD was reduced, catabolic ability of single carbon source was declined too. No obvious influence of O₃ combined with shading on soil microbial diversity was found. Principal Component Analysis suggested that different forms of carbon source have different sensitivity. The variation degree of carbohydrates' is higher than others. Under ozone fumigation combined with reduced solar irradiance, AWCD and catabolic ability of single carbon sources were lower than enhanced O₃ or lower solar irradiance single acting. For soil microbial, two years' results showed that combination effects of ozone fumigation and reduced solar irradiation reduced both rate and total utilization of carbon sources except polymers. However, there was no direct impact on the diversity of soil microbial. For Inhibitory effect on the degradation of carbon, combined effect of enhanced ozone concentration and reduced solar irradiation was greater than each factor functioning alone. 20% reduction of solar radiation increased the decomposition of polymers to some extent. On the conditions of combined O₃ fumigation and solar radiation weakened, that makes the metabolism of carbohydrates and amines highly variable, as they are largely affected by the environment. Enhanced O₃ and lower solar irradiation have a cooperative action. Reduced solar irradiance is benefit for winter wheat absorbing O₃, resulting in a serious injury.