Global climate change is a key factor which leads to either a decline or an extinction of amphibian populations worldwide. This paper investigates the effects of high environmental temperatures on non-specific immunity and energy consumption in the male toads (Bufo bufo gargarizans). We found that peripheral blood cell phagocytosis decreases while spleen macrophage respiratory burst activity transiently increases in high-temperature toads (32 ℃) versus control toads (22 ℃). Blood glucose levels decrease in high-temperature toads after 24 h and 48 h exposure versus control toads, while hepatic glycogen content remains unchanged. In addition, the ATP content in the high-temperature toad testis gradually increases and reaches significant levels after 24 h and 48 h exposure versus control toads. Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activity show no significant changes. Our results indicate that high environmental temperatures cause a decline in immunocompetence, an increase in glucose consumption, and an accumulation of energy (ATP) within the testis in Bufo bufo gargarizans.