In this paper, more Qilian juniper (Sabina przewalskii Kom.) tree ring samples in 12 sites from different ecological environment in Qinghai Province are collected, and the 12 tree ring chronologies are developed using three different detrending methods, contain spline function detrending method(SPL), negative exponential fitting detrending method(NEP) and regional curve detrending method(RCS). Combined temperature and precipitation data from 30 Meteorological stations in Qinghai, the Qilian juniper radial growth responses to climate in the different ecological environment and detrending method were analyzed using correlation function and the response function method. The results showed that the average temperature before trees growing season is a major limiting factor on Qinlian Mountains, and there have a good correlation between the NEP tree-ring width chronologies and the average temperature in last winter. In the Qaidam Basin, The precipitation in growing season is the limiting factor for tree radial growth, there have a better correlation between the SPL tree-ring width chronologies and the precipitation. On the southern Qinghai, the Qilian juniper radial growth response to average temperature in May, there is most sensitive, while the SPL tree-ring width chronologies and temperature in May showed a significant negative correlation, and the correlation coefficient exceed -0.606. In the eastern region of Qinghai, Qilian juniper tree radial growth response to climate generally not sensitive. There have a good correlation between Qilian juniper radial growth and North Atlantic Oscillation Index(NAO)/Arctic Oscillation Index (AO) in Qaidam Basin, and there also have some relationship on Qilian Mountains. There have a good correlation between Qilian juniper radial growth and India-Burma trough Index(IBT) on southern Qinghai Plateau, and tree-ring chronologies correlates better with the Pacific Decadal Oscillation(PDO) in the eastern region of Qinghai. The results showed that the tree-ring chronologies significantly affected by the westerly climate in Qaidam Basin and Qilian Mountains, and the Indian monsoon dominate to Qilian juniper radial growth on southern Qinghai Plateau. In the eastern Qinghai, Qilian juniper radial growth affected by the East Asian monsoon.