The breeding of the inbred line is the basis of studying the characteristics of animal genetics. We studied the genetic basis of alien invasive species-Bursaphelenchus xylophilus, and established the inbred lines of two local populations of China(CN-ZJ) and the United States (US-7H), and compared the differences of fertility before and after the breeding of the inbred lines. Eight strains were established in the inbreeding of US-7H population and 4 strains were established in the inbreeding of CN-ZJ population. There was no effect of inbreeding on the fecundity of pine wood nematode. The population fecundity of CN-ZJ is better than that of the US-7H population. The breeding of the inbred lines paves the rode for further research about genetic mechanism on the invasion of Bursaphelenchus xylophilus.