A field survey was conducted to determine the effect of available burrow densities of plateau pika with 10, 15, 21 and 31 per 625 m² on leaf nitrogen (N) and phosphorus (P) stoichiometry of dominant plants and soil of alpine meadow by using available burrow density substituting for activity degree of plateau pika in this study. The dominant plant included Kobresia pygmaea, Elymus nutans and Anemone rivularis var. flore-minore. This study showed that the leaf N content of K. pygmaea, E. nutans and A. Rivularis significantly increased as the available burrow density increased (P < 0.05). The leaf P content of K. pygmaea and E. Nutans firstly increased and then significantly decreased (P < 0.05), and leaf P of A. Rivularis increased gradually (P < 0.05) with the increase of available burrow density. The leaf N:P was different among three dominant plants, indicating that the leaf N:P of K. pygmaea significantly decreased (P < 0.05), and the leaf N:P of E. nutans increased gradually, but that of A. Rivularis firstly increased and then decreased as the available burrow density increased. This study also showed that the soil N content at 0-10 cm and at 10-20 cm layer was similar as the available burrow density increased. The soil P content at 0-10 cm layer firstly decreased and then increased, and that at 10-20 cm layer decreased with the increase of the available burrow density. The soil N:P at 0-10 cm layer was similar and that at 10-20 cm layer increased gradually in the increasing process of available burrow density. This study suggested that the correlation relationship between leaf N, P, N:P and soil N, P, N:P was regulated by distribution features of plant root systems and microhabitat where plants grow.