By selected two standard runoff plots of Mixed conifer (Pinus massoniana × Gordonia acuminata) and Evergreen broad-leaved forest (Gordonia acuminata × Symplocos setchuanensis) in Jinyun Mountain, combined with field observations and laboratory analysis of rainfall and runoff chemical properties, conducts a study about the runoff variation of base cations in the hydrological process to provide a basis for the protection of the water environment in this region. The results show that: (1) pH value in Mixed coniferous and evergreen broad-leaved forest have the same trends: surface runoff > interflow> precipitation. There is significant correlation between pH and rainfall.(2) In rainfall-surface runoff-interflow process, the sum of NH₄⁺ surface runoff output and interflow output is less than the amount of rainfall input, indicating that the soil have a certain of retain the ability of NH₄⁺ to make it loss less. While the runoff outputs of other base cations are higher than rainfall input and element loss, respectively. In terms of the total amount of base cations, compared to the rainfall the runoff output of base cations increased:mixed coniferous (2.86 kg/hm²), evergreen broad-leaved forest (2.47 kg/hm²), which indicates that there is a loss of base cations in the evergreen broad-leaved forest and the mixed coniferous.(3) It has correlation between the runoff output of base cations (K⁺, Ca²⁺, Mg²⁺ and Na⁺) and the amount of runoff, respectively, which mean the output of cations rise with the increase of runoff. While that of NH₄⁺ has no obvious correlation between the output runoff. In addition to the impact of runoff, the changes of NH₄⁺ may also be affected by other factors such as micro-organisms, season and so on.