To determine the changes in soil denitrification and N₂O emission from crop rhizosphere under ozone (O₃) stress and the declined solar irradiance, ozone treatments in OTCs (open top chambers) and solar irradiance manipulate in shading nets were used. The effect of increased atmospheric surface O₃ level and declined solar irradiance with their combination contents on soil denitrification in rhizosphere of winter wheat (Triticum aestivum)was thus investigated, during the growing/development (G/D) periods including reviving, jointing, booting, heading, filing and mature stages. And four experiments were performed during a four years experiment by testing once in each year,using six OTCs or six shaded chambers, and OTCs combine with shading net in each, respectively. The results showed that each OTC was equivalent, but two were fed with air while the other four with ozone at different concentration, of which two chambers were 100 nL/L and the other two were 150 nL/L. Shaded chambers were found to be equivalent either, they were all shading but with uent flow, solar irradiance was found to be reduced by 20%,40%,60%, respectively, when tests in each chamber were repeated twice. The unshaded treatment was found to be contrasts. Complex-action of O₃ and declined solar irradiance were tested by shading net over OTCs, where winter wheat was grown to all life stage in shading net and combine chambers. From reviving to mature stages, the plants were treated for 8 h (8:00-16:00) in every sunny day with different dose of O₃, respectively in OTCs of shading chambers covered with net all life stage. Soil amount of denitrifying bacteria and denitrifying capacity were measured, with each treatment assayed thrice. Data were initially compared by analyzing the variance and differences between means using the Duncans Multiple Range Tests. Values of P < 0.05 was set to indicate significance. Quite similar results were obtained during four years' experiments, suggesting that when a G/D stage is progressing, soil denitrifying bacteria remains at similarity level. Untill mature stage that is postharvest, it rises up sharply. Without enhanced O₃ or lower solar irradiance condition, soil denitrifying capacity were found to decline firstly but consequently increased. Under O₃ stress, denitrifying capacity gradually increased. After ozone fumigated continuously during three growing season it increased significantly, compared to the control, but the soil N₂O emission risk was heighten at the same time. O₃ combine with the shading treatments' soil denitrifying bacteria and capacity were found to be induced in every growing/development (G/D) periods of winter wheat, while the promoting rate were higher than O₃ or shading separate role. The results indicated that the enhanced O₃ and lower solar irradiance can improve soil denitrifying process, with cooperative relations. Lower solar is irradiant to the benefit of O₃ absorb, to O₃ damages increase and to facilitate denitrifying process.