Abstract:The oriental fruit moth (OFM), Grapholita molesta Busck is one of the major fruit tree pest insects which lay their eggs on the leaves of peach young shoots. In order to find out the roles of the host colour in oviposition orientation, we conducted a comparative study to reveal effects of 12 different colours (red, pink, light pink, orange yellow, deep yellow, light yellow, green, deep green, light green, blue, purple, brown etc) on oviposition preference of adults using the color cardboard to simulate the host colours outdoors. The results have showed that colour affected the oviposition preference significantly. OFM preferred to the light yellow and light green laying eggs, with oviposition preferences of 68.9% (white reference) and 63.8% (black reference) on light yellow cards, 64.1% and 65.5% on light green cards. OFM showed a certain avoidance to blue and light pink cards, with oviposition preferences of 47.7% and 40.4% on blue cards, 47.2% and 42.7% on light pink cards. and the referance colours can significantly affect the oviposition preference. Colour also affected the fecundity significantly. whether while or black referance, the eggs spawed on yellow and green cards were much more than others, especially the deep yellow, deep green and green cards. Colour affected the oviposition preference of 1-7 days adults significantly. 2 and 3 days of OFM preferred to the light yellow and light green laying eggs on white reference, with oviposition preferances of 86.7% and 91.7%. On black reference, 2 day moth preferred light green laying eggs, with oviposition preferance of 79.7% and 6 day moth preferred light yellow, with oviposition preferance of 74.8%. The study showed that host colour play an important role in the spawning site selection, and provide bases and references for the application of colour in the monitoring and prevention and control of egg stage and even further reveals the spawning host selection mechanism of OFM.