薇甘菊萎蔫病毒感染对薇甘菊光合特性和4种酶活性的影响
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华南农业大学亚热带农业生物资源保护与利用国家重点实验室,华南农业大学亚热带农业生物资源保护与利用国家重点实验室,华南农业大学亚热带农业生物资源保护与利用国家重点实验室,华南农业大学亚热带农业生物资源保护与利用国家重点实验室,华南农业大学亚热带农业生物资源保护与利用国家重点实验室,华南农业大学亚热带农业生物资源保护与利用国家重点实验室,华南农业大学亚热带农业生物资源保护与利用国家重点实验室

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国家自然科学基金项目(31000260, 31100286, 31028018); 广东省自然科学基金项目(S2011040004336)


Effects of Mikania micrantha wilt virus infection on photosynthesis and the activities of four enzymes in Mikania micrantha H.B.K.
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State Key Laboratory for Conservation and Utilization of Subtropical Agricultural Bio-resources,,,,,,State Key Laboratory for Conservation and Utilization of Subtropical Agricultural Bio-resources

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    摘要:

    为明确薇甘菊萎蔫病毒(Mikania micrantha wilt virus, MMWV)对薇甘菊(Mikania micrantha)叶片生理生化的影响,探讨了MMWV侵染对薇甘菊叶片超氧化物岐化酶(superoxide dismutase, SOD)、过氧化物酶(peroxidase, POD)、多酚氧化酶(polyphenol oxidase, PPO)和苯丙氨酸解氨酶(phenylalanine ammonia-lyase, PAL)活性及叶绿素总含量和光合特性的影响。结果表明: MMWV感染薇甘菊16d内叶片的SOD 活性均比对照组高, 其中第16天达最大值,但接种后第24和32天SOD 活性分别比对照低了13.28%和25.37%;POD活性在接种后16-32d均显著高于对照。PPO和PAL变化趋势相似,在接种后第8天这两个酶的活性分别比对照高了77.75%和23.58%,而在第32天分别比对照减少了14.27%和20.53%。随着侵染时间的增加,感病薇甘菊叶片中叶绿素含量减少,叶绿素a/b值逐步降低; 同时最大净光合速率(Pmax)和光饱和点(LSP)分别比健康对照减少了32.34%和12.52%,而对暗呼吸速率(Rd),光补偿点(LCP)和表观量子效率(AQY)无显著影响。表明MMWV侵染可减低薇甘菊叶片光合作用的效率。

    Abstract:

    Mikania micrantha (Compositae) is one of the world's most aggressive weeds and is responsible for substantial damage caused to natural ecosystems and biodiversity in South china. A new virus infecting Mikania micrantha, designated Mikania micrantha wilt virus (MMWV), belongs to a third subgroup of the genus Fabavirus and represents a distinct new isolate of Gentian mosaic virus (GeMV). Although MMWV is not lethal to M. micrantha, MMWV efficiently inhibits the growth of its host under both laboratory and field conditions. The feasibility of using MMWV to control M. micrantha is therefore worth exploring further. To gain further understanding concerning the physiological and biochemical effects of MMWV infection on Mikania micrantha, the activities of superoxide dismutase (SOD), peroxidase (POD), phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), as well as chlorophyll a/b ratios, total chlorophyll content, and photosynthetic activity were evaluated in M. micrantha leaf tissues following MMWV infection. In comparison with uninfected control leaves, our results revealed that chlorophyll a, b and total chlorophyll contents, and the ratio of chlorophyll a/b decreased by 30.76%, 18.76%, 26.41% and 17.84%, respectively, by 32 d post-MMWV infection. SOD activity significantly increased and reached maximal levels within 16 d post-infection, however activities at 24 and 32 d were 13.28% and 25.37% lower than those of control (uninfected) plants, respectively. POD activities were significantly higher in leaves of inoculated plants relative to control plants throughout the experimental period, with maximal levels occurring by 24 d post-infection. POD activities at 16, 24 and 32 d post-infection were increased by 16.70%, 41.12% and 16.11%, respectively, relative to levels observed in control plants. The activity of PPO in infected M. micrantha leaves reached maximal levels at 8 d post-infection, increasing to 77.75% above control levels, however PPO levels were 14.27% below control levels by 32 d. PAL activities following MMWV infection exhibited a pattern similar to that observed for PPO, increasing to 23.58% above control levels, then subsequently declining to 20.53% below control levels, at 8 and 32 d day post-infection, respectively. Maximal net photosynthetic rate (Pmax), dark respiration rate (Rd), light compensation point (LCP), light saturation point (LSP) and apparent quantum yield (AQY) were 11.76 μmol·m-2·s-1,-2.23 μmol·m-2·s-1, 42.56 μmol·m-2·s-1, 1063.92 μmol·m-2·s-1 and 0.04 μmol/μmol, respectively,in MMWV-infected leaves at 32 d post-infection. In contrast, Pmax, Rd, LCP, LSP and AQY values were 17.37 μmol·m-2·s-1,-2.43μmol·m-2·s-1, 36.21 μmol·m-2·s-1, 1216.13 μmol·m-2·s-1 and 0.05 μmol/μmol, respectively, in uninfected leaves at 32 d. In comparison with control leaves, Pmax and LSP values declined by 32.34 and 12.52% following MMWV infection, however, Rd, LCP and AQY values showed no significant change during the experimental period. Our results provide key information concerning the reduction of photosynthetic efficiency in leaves of M. micrantha associated with MMWV infection.

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王瑞龙,潘婉文,杨娇瑜,张晖,夏晴晴,苏贻娟,曾任森.薇甘菊萎蔫病毒感染对薇甘菊光合特性和4种酶活性的影响.生态学报,2013,33(5):1667~1674

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