Most insects can mate multiple times. With the increasing of male mating frequency, male parasitoid wasps would have less sperm, and the female partners would gain less sperm during copulation, which will make them lay more unfertilized eggs, developing into male offspring. Some solitary female parasitoid wasps can only mate once in the life span. In this article, the mating behavior of the solitary endoparasitoid, Pachycrepoideus vindemmiae Rondani, was described, with the pupae of houseflies as the hosts, and the effects of male mating frequency on the production of the female partners and the possible mating times of the mated females were studied. The results showed that the mating behavior of P. vindemmiae included courtship, precopulatory, copulation, and postcopulatory, which lasts (41.21±83.16) s, (26.05±17.99) s, (2.62±0.62) s, and (30.43±11.44) s, respectively. When mated with males for the 1^st, 3^rd, 6^th, 9^th, 12^th, and 15^th times, the longevity of the females was (11.18±7.17) d, (10.82±8.51) d, (9.09±5.74) d, (9.36±4.74) d, (9.64±3.47) d, and (13.45±7.03) d, respectively, and the oviposition duration was (9.73±7.52) d, (9.82±8.51) d, (7.55±5.20) d, (8.18±4.71) d, (8.55±3.50) d, and (11.64±7.50) d, respectively. The total progeny number was (57.00±39.15), (50.18±35.52), (44.18±24.62), (42.55±15.69), (47.27±18.23), and (62.09±37.55), respectively, and the male proportion was (0.21±0.19), (0.17±0.06), (0.27±0.22), (0.43±0.31), (0.45±0.30), and (0.63±0.34), respectively. The mating frequency of males did not significantly affected longevity, oviposition duration and total progeny number of the female partners, but significantly affected the production of female and male progeny and offspring sex ratio, respectively. With the increasing of male mating times and with the increasing of female oviposition duration, daily female offspring number per foundress decreased, but male offspring number increased. When males mated for the 1^st, 3^rd, 6^th, 9^th, 12^th, and 15^th times, females laid offspring with male proportions higher than 50% on the 8^th, 17^th, 11^th, 6^th, 5^th, 2^nd day, respectively. The interaction of female ages and mating frequency affected offspring sex ratio significantly. After mated with the males which had mated multiple times, females laid more male offspring earlier, which suggests the sperm limitation in females was more obviously when they had mated with multiply mated males. Mated females could not finish mating again neither just after mating nor after they had laid eggs for 8 days, which suggests females might mate only once in their life span, no matter whether they lack sperm or not.