Rising tropospheric ozone concentration constrained plant growth and thus result in yield loss of rice. However little is known about response of growth and yield of rice to elevated ozone concentration under different cultivation measures. By using an advanced FACE (Free Air gas Concentration Enrichment) system, hybrid rice cultivar shanyou 63 was grown at ambient (A-O₃) and elevated ozone concentrations (E-O₃,ambient×1.5). At transplanting, week seedlings (no tillers) and robust seedlings (with 2 tillers) were selected and planted with 3 spacing levels: low plant density(16 hills/m²), medium (24 hills/m²) and high plant density(32 hills/m²). Results showed as follows: Ozone stress decreased the SPAD value, net photosynthetic rate (P_n), stomatal conductivity (G_s) and transpiration rate (T_r) of flag leaves, but there was no significant effect on intercellular CO₂ concentration(C_i) or leaf temperature(T_l). There was no significant ozone effect on dry matter production before jointing stage. However, elevated significantly reduced dry matter production from jointing to heading stage and from heading to maturity stage by 13% and 29%, respectively. As a result, reduced dry matter accumulation at maturity stage and significant yield reduction were observed at harvest. The analysis of variance showed that seedling quality by O₃ interaction was not significant, but significant interactions were detected between O₃ and plant density for yield. The above results indicate that ozone stress significantly inhibited photosynthesis of rice during late growth stage, which result in reduced dry matter production and yield loss of rice. Ozone-induced yield loss could be mitigated by appropriately increasing plant density.