湿地芦苇根结合好气细菌群落时空分布及其与水质因子的关系
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首都师范大学资源环境与旅游学院;资源环境与地理信息系统北京市重点实验室,首都师范大学资源环境与旅游学院;资源环境与地理信息系统北京市重点实验室,首都师范大学资源环境与旅游学院;资源环境与地理信息系统北京市重点实验室

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国家自然科学基金(40901281)


Spatial-temporal variation of root-associated aerobic bacterial communities of phragmites australis and the linkage of water quality factors in constructed wetland
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College of Resources Environment and Tourism,Capital Normal University,College of Resources Environment and Tourism,Capital Normal University,College of Resources Environment and Tourism,Capital Normal University

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    摘要:

    再生水作为河湖补充水已经成为再生水应用及城市水生态环境改善的重要方面,但是再生水富含营养元素极易导致水体富营养化。湿地微生物作为再生水净化的主要参与者在再生水净化及再生水补水湿地水生态恢复方面发挥重要作用。以北京奥林匹克公园再生水人工湿地净化系统为例,利用ARDRA技术分析湿地净化系统中芦苇根结合好气细菌群落结构的时空分布特征,并借助CCA排序的方法揭示根结合好气芦苇细菌群落结构与水环境因子的关系。结果表明:人工湿地净化系统芦苇根结合好气细菌群落结构季节差异明显,夏季较秋季细菌群落密度增加,群落多样性增高,均匀度增大;与秋季相比,夏季净化系统各处理区细菌群落结构差异较大即各处理区好气细菌优势类群变异较大,偶见类群频度较高,而秋季净化系统中各处理区优势类群趋同,偶见类群频度较低。无论夏季还是秋季空间变异规律均表现为潜流湿地较氧化塘更有利于微生物群落密度增高及细菌群落多样性增加,其季节变异较氧化塘更为显著;与循环水处理湿地相比,再生水处理湿地受进水水质的特有属性影响,具有最高群落密度与群落多样性。环境解释结果表明:芦苇根结合好气细菌类群OTU1、OTU28在净化系统中具较稳定的丰度,是一些生态幅度比较广泛的种属,受水质变量影响较小,在细菌群落的物质和能量代谢中起重要作用,对人工湿地净化系统稳定性具重要贡献。水质变量NH4+、TN、ORP、TP是芦苇根结合好气细菌群落结构季节差异的驱动因子,水质变量NO3-、TDS、pH、Sal则是导致夏季细菌群落结构空间差异的关键因子。

    Abstract:

    Reclaimed water as supplemental source of urban rivers and lakes is regarded as an important approach to improve urban aquatic ecological environment. However, high concentration of nitrogen and phosphorus from reclaimed water can easily lead to eutrophication. Bacteria in constructed wetland are known to play an important role in aquatic ecological remediation. The community structure and diversity of cultural associated bacteria in roots of reed (phragmites australis) growing in reclaimed water management system (reclaimed water treatment unit, cycling water treatment unit, oxidation ponds) in Beijing Olympic Park was investigated in summer and autumn by 16S rDNA cloning, Amplified Ribosomal DNA Restriction Analysis (ARDRA), and sequence homology comparison. Canonical Correspondence Analysis (CCA) and Two way Indicator Species Analysis (TWINSPAN) were used to estimate and statistical test the effects of environmental variables and other explanatory variables on bio-communities, especially to unravel the simultaneous response of the species in a bacteria community to environmental variations when the effects are hidden by other large sources of variation. Cluster analysis on bacteria clones showed that 29 operational taxonomic units (OTUs) were identified based on the similarity of ARDRA banding profiles in 328 cultural clones. Environmental changes resulted into a seasonal shift of bacteria community on community density and diversity in reclaimed water management system. Community density and evenness of root-associated aerobic bacteria in summer increased 76.37%-96.70% than those in autumn. Kinds of inflow (reclaimed water or cycling water) and types of matrix both contributed to spatial variability of bacterial community. There was relative high community diversity and density with reclaimed water inflow and IVCW matrix in reclaimed water treatment unit. Spatial variability of bacteria community in summer increased with altering dominant species in each unit and more accidental species. Therefore, reclaimed water treatment unit in summer with the highest community density (29.9×107CFU/g roots weight) and the Shannon-Weaver (H'=1.824). CCA and TWINSPAN analysis revealed that OTU1 and OTU28 in constructed wetland system were with the wide ecological amplitude, which contributed to the stability of constructed wetland system and hardly disturbed by water viabilities, played an important role in material metabolism and energy metabolism. NH4+、TN、ORP、TP in water drived seasonal variability of community structure of reed root-associated aerobic bacteria, while NO3-、TDS、pH、Sal in water leaded to spatial variability community structure of reed root-associated aerobic bacteria of summer reclaimed management system.

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熊薇,郭逍宇,赵霏.湿地芦苇根结合好气细菌群落时空分布及其与水质因子的关系.生态学报,2013,33(5):1443~1455

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