It has been reported that the EP-1, a contraceptive compound composed of levonorgestrel and quinestrol with the ratio of 1:2, has negative effects on the breeding and reproductive behaviors in some species of mice and rats. However, little is known that the effects of EP-1 on spatial memory and anxiety in mice. Here, the effects of treatment time (15 d and 30 d) and dose (0 mg/kg (control), 1.0 mg/kg, 2.0 mg/kg, 3.0 mg/kg) of EP-1 on spatial memory and anxiety in Mus musculus were tested using Morris water maze and elevated plus maze separately. Negative effects of 2.0 mg/kg EP-1 on spatial memory of the subjects were found in 15 days, and this ability recovered in 30 days, implying the 2.0 mg/kg EP-1 can decrease the ability of spatial memory of M. musculus within a period of time. No significant effects were found in all treatments of time and dose of EP-1 on anxiety of the subjects. The results might help to evaluate the effects of EP-1 on mice in respect to non-reproductive behaviors.