The output of natural eco-service function is eco-service efficiency; it can be divided into positive and negative eco-services. Rapid urbanization occupies amounts of non-construction areas that are important for maintaining local favorable environment; this process affects the natural eco-service functions, and results in deterioration of city's eco-environment, in other words, the negative eco-service efficiency increases. As a typical rapidly urbanization city at the eastern of China, Changzhou's urbanization occupied lots of lands for eco-services, it caused many environmental problems. Under the support of GIS and RS technology, this paper used methods of grid analysis and complex ecological assessment, and established the spatial indexes system for complex eco-services efficiency assessment from both positive and negative eco-services of the current land use. The results show that there is about 64.5 percent land's complex eco-service is positive, and 35.5 percent land's complex eco-service is negative. According to the city's current land use planning trend, it would result in more lands changing from positive eco-services into negative efficiency. The results provide a scientific reference for decision-making of local land use planning and management for urban future development.