A pot experiment was conducted to investigate impact of magnesium (Mg), manganese (Mn), activated carbon and lime and their interactions on wheat growth and cadmium (Cd) uptake from a Cd polluted agricultural soil. Results showed that application of appropriate amounts of magnesium sulfate (Mg), manganese sulfate (Mn) or in combination with lime could promote wheat growth and seed yields, while addition of lime alone or in combination with activated carbon significantly reduced wheat seed yield. Application of Mg significantly reduced concentrations and uptake of Cd in wheat seeds and straw and positively related to Mg rates. Concentrations and uptake of Cd in wheat seeds and straw were effectively decreased by the lower rates of Mn but increased by the higher rates. Lime and activated carbon, no matter it was used alone or together, remarkably restricted Cd uptake by wheat, while Cd ratio of seed/straw tended to increase with lime rates. Folia spray of Mg was equally effective as incorporating Mg into soil on restricting Cd uptake by wheat but a significant depressing effect on Cd uptake by folia spray of Mn over soil application of Mn was observed. Whether Mg and Mn or lime, Mg and Mn were used together produced pronounced positive interactions on depressing Cd concentrations and uptake in wheat seeds and significant restrictive effect on Cd transfer from straw to seeds as well.