不同增温处理对夏蜡梅光合特性和叶绿素荧光参数的影响
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西南大学三峡库区生态环境教育部重点实验室,台州学院生态研究所,中国科学院植物研究所植被与环境变化国家重点实验室,杭州师范大学生命与环境科学学院,广西师范大学生命科学学院

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国家自然科学基金项目(30870392); 浙江省自然科学基金项目(Y3080460)


Effects of different day/night warming on the photosynthetic characteristics and chlorophyll fluorescence parameters of Sinocalycanthus chinensis seedlings
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Key laboratory of Eco-environments in Three Gorges Reservior Region,Ministry of Education School of Life Sciences,Southwest University;China;Institute of Ecology,Taizhou College;China;State Key Laboratory of Vegetation and Environmental Change,Institute of Botany,Chinese Academy of Sciences;China;College of Life and Environment Sciences, Hangzhou Normal University, China;College of Life Sciences, Guangxi Normal University, China,Institute of Ecology,Taizhou College,State Key Laboratory of Vegetation and Environmental Change,Institute of Botany,Chinese Academy of Sciences,College of Life and Environment Sciences, Hangzhou Normal University,College of Life Sciences, Guangxi Normal University

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    摘要:

    利用红外线辐射器(Electric radiant infrared heater)设置3个不同增温处理:(1)晚上增温2 ℃(18:00-翌日6:00);(2)白天增温2 ℃(6:00-18:00);(3)全天增温2 ℃;以不做增温处理作为对照,模拟气候变暖对夏蜡梅(Sinocalycanthus chinensis)的光合特性和叶绿素荧光参数的影响。3个季节中,3种增温处理下的夏蜡梅净光合速率(Anet)和最大净光合速率(Amax)均高于对照。表明3种增温处理均可以提高夏蜡梅的光合作用能力。增温处理提高了气孔导度(Gs)、暗呼吸速率(Rd),降低了胞间CO2浓度(Ci)。3种增温处理中,夏蜡梅在3个生长季节的AnetAmax均以白天增温最大,表明白天增温下夏蜡梅具有最高的光合作用能力。白天增温处理下的表观量子效率(AQY)、光饱和点(LSP)显著高于全天增温、晚上增温以及对照。白天增温和晚上增温使夏蜡梅的相对叶绿素含量显著增加,且白天增温叶绿素相对含量显著高于晚上增温、全天增温及对照。白天增温、晚上增温与全天增温处理下的夏蜡梅的PSⅡ最大光化学效率(Fv/Fm)均显著低于对照,在12:00和14:00时,三者之间存在显著性差异,以白天增温最高,晚上增温次之,全天增温最低。总之,3种增温处理夏蜡梅的光合能力总体上均有不同程度的增强,且在白天增温处理下,夏蜡梅的AnetAmax值最高,表明白天增温下夏蜡梅具有最高的光合能力,这与白天增温引起的相对叶绿素含量增高、叶片对强光的适应能力加强、较低程度的光胁迫等因素有关。本研究说明未来增温条件下,将提高夏蜡梅的光合生理活性,促进夏蜡梅的生长。

    Abstract:

    Global temperatures are now predicted to be 1-6 ℃ warmer than today by the year 2100. Environmental warming is likely to have significant effects on plant carbon relations, particularly through its effects on photosynthesis and respiration. In terrestrial environments, the nighttime daily minimum air temperatures in recent decades have increased more than the daytime maximum air temperatures. Understanding how plants respond to differential day/night warming may help scientists to predict the responses of plants to global warming. Most published studies of the effects of warming on plant growth have focused on either dominant species or invasive species. Little attention has been paid to endangered plants, but understanding how such species adapt in the context of global warming may be critical to their survival. Sinocalycanthus chinensis is an endangered plant belonging to the family Calycanthaceae. Here, we analyzed the effects of differential simulated day/night warming on the photosynthesis characteristics and chlorophyll fluorescence parameters of S. chinensis seedlings. Three different simulated day/night warming treatments were included in the experiment: 1) warming of 2 ℃ above ambient temperature at daytime; 2) warming of 2 ℃ at nighttime; 3) warming of 2 ℃ at both day- and nighttime. Seedlings exposed to ambient temperatures without warming were used as the control treatment. In three seasons, the net photosynthetic rate (Anet) and the maximum net photosynthetic rate (Amax) in all three warming treatments were higher than in the control, indicating that simulated warming could increase the photosynthetic ability ofS. chinensis. Simulated warming also increased the daily mean stomatal conductance (Gs) and the dark respiration rate (Rd), while it decreased the daily mean intercellular concentrations of CO2. In three different seasons, Anet and Amax ofS. chinensis seedlings subjected to daytime warming were the highest, indicating that S. chinensis seedlings with daytime warming had the highest photosynthetic ability. The apparent quantum yield, light saturation point, and relative chlorophyll content of S. chinensis seedlings subjected to daytime warming were significantly higher than plants in the other warming treatments and the control. Simulated warming significantly decreased the Fv/Fm value of S. chinensis seedlings. During the period from 12:00 pm to 2:00 pm, the Fv/Fm value of S. chinensis seedlings treated with daytime warming was the highest, followed by those treated with nighttime warming and all-day warming, with significant statistical differences among the treatments. In conclusion, the different warming treatments increased the photosynthetic ability of S. chinensis to different degrees. The maximum values of Anet and Amax of S. chinensis seedlings subjected to daytime warming suggested that the highest photosynthetic ability of S. chinensis seedlings occurred under daytime warming. This might be due to the increase in relative chlorophyll content, the enhancement of the light adaptation ability, and the lower levels of light stress in the leaves of S. chinensis seedlings under daytime warming. The results of this study suggested that warming conditions in the future will improve the photosynthetic physiological activity and promote the growth of S. chinensis, perhaps benefiting this endangered species.

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徐兴利,金则新,何维明,王兴龙,车秀霞.不同增温处理对夏蜡梅光合特性和叶绿素荧光参数的影响.生态学报,2012,32(20):6343~6353

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