Abstract:Leaf area index (LAI), defined as the total one-sided leaf area per unit ground surface area (m2m-2), is among the most frequently used parameters for analysis of plant canopy structure. Most ecosystem process models that simulate carbon and water cycles at a stand or regional scale require LAI as an input variable, thus accurate measurement of LAI is essential for conversion of leaf-level processes to the canopy level. The main methods of LAI measurement generally are classifiable into two groups: direct measures, which mainly include destructive sampling and the litterfall method; and indirect measures, which mainly include hemispherical photography and the LAI-2000 plant canopy analyzer. However, using a direct or indirect method alone may not accurately estimate LAI for mixed needle and broadleaf forest or broadleaf forest with a high proportion of evergreen species. Secondary Betula platyphylla is among the most common broadleaf forest types that regenerate naturally after clear cutting of broadleaved-Korean pine forest in the Xiaoxing'an Mountains in north-east China. In the present study, we estimated LAI and its seasonal dynamics in secondary B. platyphylla forest in the Xiaoxing'an Mountains using optical methods (hemispherical photography and the LAI-2000 Plant Canopy Analyzer) and a combinational method (hemispherical photography combined with the litterfall method). In the combinational method, hemispherical photographs obtained in early November were calibrated with consideration of the woody-to-total ratio (α) as well as the clumping index (for clumping beyond the shoots, ΩE). The calibrated value was regarded as the true LAI (LAIt) of coniferous species; the LAIt of the study stand and its seasonal dynamics (from July to November) were obtained by supplementation of the LAIt of coniferous species by incorporation of litterfall data for each observation. Using this result as a reference value, we compared the results obtained with the two optical methods. The LAI for the litter of B. platyphylla and Larix gmelini accounted for 87.65% of the total litter, and leaves of B. platyphylla began to fall distinctly earlier than those of other species, and the LAI for the litter of B. platyphylla accounted for 98.24% of all species during July and August. Leaf fall for most species excluding B. platyphylla peaked in mid-September. In contrast to the proposed method, hemispherical photography and LAI-2000 both underestimated LAI by 2.83% and 6.20%, respectively, during the maximum LAI period and overestimated LAI significantly on average by 118.13% and 89.34%, respectively, on other dates. However, hemispherical photography and LAI-2000 yielded LAI values that were significantly correlated with the results obtained with the combinational method; the regression curves were LAIt= -1.1393 + 1.0934·LAIHP (R2= 0.80) and LAIt= -0.1712 + 0.6259·LAILAI-2000 (R2= 0.83), respectively. The corrected hemispherical photography data were used to simulate the seasonal dynamics of LAI from May to November, which was indicated to be accurate (R2= 0.87). The LAI estimate obtained with the two optical methods did not differ significantly from July to September, but the estimate with the LAI-2000 method was significantly lower than that obtained with hemispherical photography after October. However, LAI estimated with the two optical methods was significantly correlated (R2= 0.86) for secondary B. platyphylla forest. This study lays a foundation for convenient, rapid and accurate estimation of LAI for secondary B. platyphylla forest in subsequent studies.