The four populations of Fomes fomentarius from Maoer Mountain, Changbai Mountain, Liangshui and Benxi were studied. The growth rates of F. fomentarius from four locations were compared by measuring the hypha growth in the PDA plate at 23℃ and 28℃, as well as the biomass by means of the mycelium dry weight in the PDA liquid medium at 23℃. The results displayed that the average growth rate of the fungi from four locations was 0.9cm/d at 23 ℃, and 0.64cm /d at 28 ℃. The growth rate of F. fomentarius at 23℃ was notably faster than that at 28℃ in the PDA plate, which indicated that the growth of this fungal species was sensitive to the temperature, for that 23℃ was more appropriate. F. fomentarius from different locations had no significant difference in growth rate at the same incubation temperature. So did the biomass of the fungi. The activities of three main lignin degradation enzymes (laccase, lignin peroxidase, manganese peroxidase) produced by F. fomentarius were detected by colorimetry. The results showed that the activity of three lignin enzymes produced by F. fomentarius increased gradually during 12 day incubation. There was significant differences in the enzyme activities cultured in different media, in which the activity of three enzymes in the PDA medium were significantly higher than that in complete medium. And also the activity of laccase was in the highest, manganese peroxidase in the second and lignin peroxidase in the lowest. The activity of the same enzyme from different locations had no significant differences. The sequence-related amplified polymorphism (SRAP) was used to evaluate genetic diversity and genetic differentiation among four populations of F. fomentarius. The results showed that Benxi population had the highest genetic diversity among the four populations, followed by Maoer Mountain and Liangshui, Changbai Mountain was the lowest. AMOVA analysis revealed that the genetic differentiation was 24.74% among populations, and 75.26% within population, that meant the genetic differentiation of F. fomentarius came up intra-populations mainly. Cluster analysis by UPGMA dendrogram based on Nei's genetic distance revealed that the populations from Maoer Mountain and Benxi clustered first, Changbai Mountain population was in the second, and Liangshui population was the last. This study will provide useful information for the further research and utilization of lignin enzyme from the wood rot fungi, as well as lay the foundations of developing the engineering strains of F. fomentarius in the future.