Constructing of the city (group) ecological barrier is one of the focal points of ecological construction of China. With rapid urbanization process, urban areas, land acquisition, population and construction scale expand continuously, ecological security situation is increasingly serious, and ecological environmental protection pressure increases. The ecological barrier construction of Changzhutan(CZT) Urban Agglomeration has aroused much concern since the Urban Agglomeration was approved as an experimental area of Two-oriented Society. According to relevant principles of landscape ecology and conservation biology, based on the social research, special investigations, monographic studies and academic discussion, the pressure, demand and capacity of regional ecology were analyzed, the space level, structure, layout, function areas of ecological barrier in CZT city cluster were scientifically zoned by Modern high-tech as RS, GIS, GPS, etc., the direction and the goals of the various functional areas construction were defined, the measures of the partition management and classifying protection were proposed, the construction projects of protection planning before 2020 were programmed. The results showed that city group must arrange city space scientifically, and reserve enough ecological bases to build a strong ecological barrier preferentially based on the capacity of eco-environment, by way of ecological isolation group. In accordance with the main functional division and targeting, we must manage and arrange ecological lands strictly and rationally, strengthen plan and overall coordination in different departments, and conserve forest and wetland according to laws strictly. In addition, we must enhance public finance trans-payment and social financing efforts, carry out ecological barrier project construction earnestly, try to improve environment quality and maintain regional ecological balance to achieve the developmental goal of resource-saving, environment-friendly society with high urban quality and comprehensive competitiveness. The results may provide a scientific method and basis for urban ecological barrier construction, which also provide references for the construction of ecological barriers of other cities (city groups).