Plentiful livestock manure accumulated in large livestock field is leading to serious environmental pollution problem. Reasonable utilization of such waste has important ecological and economics significance. Decomposed cattle dung (DD) and earthworm-treated cattle dung (ED) were applied to soybean crop in order to study their influence on the growth, yield and quality of soybean. The results revealed that cattle dung treated by both methods could be able to significantly(P<0.01) promote plant height, number of branches, dry matter accumulation, add pods, seeds, seed weight per plant, soybean growth, and improve the yield and quality of soybean (DD≤30 t/hm², RD≤45 t/hm²) compared with CK. Against CK, plant height and pods per plant increased 24.2 cm and 63% respectively, in the treatment of 30 t/hm² DD; The application upper limit of organic fertilizer treated by earthworm could rise. The application of 45 t/hm² ED increased the yield and pods per plant by 72% and 75% respectively (P<0.01). The protein content and the total content of protein and fat content were the highest in the treatment of 15 t/hm² ED, which had been increased by 7% and 6% respectively, compared with CK (P<0.01). Our investigation might be useful for promoting the utilization and recycling of cattle dung in large sized livestock farms.