外源K+和水杨酸在缓解融雪剂对油松幼苗生长抑制中的效应与机理
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辽宁大学环境学院,辽宁大学环境学院,辽宁大学环境学院,辽宁大学环境学院,辽宁大学环境学院,辽宁大学环境学院,湖南农业大学资源环境学院

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国家自然科学基金(41071317);辽宁省自然科学基金(20092013);辽宁"百千万人才工程"资助项目(2008921082);沈阳市科技计划项目(F10-205-1-64);辽宁大学"211"工程第三期重点学科资助项目(HJ211001);辽宁大学省重点学科与辽宁省高校污染控制与环境修复重点实验室开放基金(HJ200907)


Alleviation effect and mechanism of exogenous potassium nitrate and salicylic acid on the growth inhibition of Pinus tabulaeformis seedlings induced by deicing salts
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School of Environmental Science,Liaoning University,School of Environmental Science,Liaoning University,School of Environmental Science,Liaoning University,School of Environmental Science,Liaoning University,School of Environmental Science,Liaoning University,School of Environmental Science,Liaoning University,School of Resources and Environment,Hunan Agricultural University

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    摘要:

    随着融雪剂在国内外寒冷地区的广泛应用及其在城市使用量的逐年增加,融雪剂对城市生态环境的危害引起了广泛的重视。其中,融雪剂在城市道路土壤中的积累对植物生长的影响已日益凸现。以油松幼苗为材料,通过分析0.2% 浓度融雪剂胁迫下外源钾(K+)和水杨酸(salicylic acid,SA)对油松幼苗各生长生理指标的影响,探讨外源K+和SA在缓解融雪剂对油松幼苗生长抑制中的机理与剂量效应关系。结果表明,0.2% 浓度的融雪剂处理对油松生长有明显的抑制作用,而20 mmol/L KNO3和2 mmol/L SA能明显诱导过氧化物酶(peroxidase,POD)活性的增强,缓解膜脂过氧化作用,降低丙二醛(malondialdehyde,MDA)在叶片中的积累,维持细胞膜的稳定性。虽然外源K+和SA对油松幼苗叶片胞间CO2浓度(intracellular CO2 concentrations,Ci)和气孔导度(stomatal conductance,Gs)的缓解作用并不显著,但其可通过提高叶绿素含量促进光合作用的进行,缓解融雪剂胁迫对油松幼苗生长的抑制,分别增加生物量24.9%和63.6%。可见,20 mmol/L KNO3和2 mmol/L SA处理能有效缓解融雪剂对油松幼苗的伤害,为城市化学融雪剂的污染防治提供科学依据。

    Abstract:

    Large quantities of deicing salts are applied in urban areas for clearing the pavement and ensuring the normal traffic flow during the winter seasons in northeastern China. The salt from deicing operation has been observed to accumulate in roadside soil and inhibit the growth of vegetation. It was reported that the application of exogenous K+ and salicylic acid (SA) could protect plants against abiotic stress. In this study, pot experiments were performed in soil culture to test the efficacy of two soil additives, potassium nitrate (KNO3) and SA, in alleviating the stress of deicing salts on growth and photosynthetic parameters of Pinus tabulaeformis seedlings. Three soil additive levels were used i.e. 10, 20, 40 mmol/L for KNO3 and 2, 4, 8 mmol/L for SA, respectively, under the stress of 0.2% deicing salts per dry weight of soil. After the treatment for 20 days (d), relative water content, leakage rates of electrolyte, malondialdehyde (MDA), peroxidase (POD) and chlorophyll content in leaves were determined. Dry weight of roots, stems and leaves were measured respectively after 60 d of treatments. Three important parameters of photosynthesis, photosynthetic rates (Pn), intracellular CO2 concentrations (Ci) and stomatal conductance (Gs) were measured every 7 d until 35 d. The results showed that the growth of seedlings was inhibited significantly by the stress of 0.2% deicing salts. The dry weight and relative water content of roots, stems and leaves under 0.2% deicing salt stress were decreased significantly in comparison with the control seedlings. The leaves of Pinus tabulaeformis were more sensitive to the stress of deicing salts compared with roots and stems. However, the treatment of 20 mmol/L KNO3 and 2mmol/L SA to 0.2% deicing salts had high dry weight and water content. Meanwhile, the MDA content and leakage rates of electrolyte increased once exposure to 0.2% deicing salts, and salts exposure also had an inhibitory effect on the activities of the POD. However, with the addition of 20 mmol/L KNO3 and 2 mmol/L SA, the high stability of membrane was improved by promoting the POD activities, with decreasing plasmalemma permeability and MDA content in leaves. On the other hand, the contents of Pn, Ci, Gs and the chlorophyll all decreased in the Pinus tabulaeformis seedlings by the treatment of 0.2% deicing salts. The addition of 20 mmol/L KNO3 significantly increased photosynthetic rates from 5.77% to 20.6% with the prolonging of the exposure time. However, other treatments were found no obvious alleviation effect. It was also found that the contents of Ci and Gs remained unchanged in leaves of Pinus tabulaeformis seedlings in all alleviation treatments, but the enhancing of chlorophyll contents played a key role in maintaining the leaf photosynthetic rate under the treatment of KNO3 and SA. The results indicated that the salt tolerance of Pinus tabulaeformis seedlings was improved by using soil additives of 20 mmol/L KNO3 and 2 mmol/L SA. Exogenous K+ and SA showed strong benefits against deicing salts-induced negative symptoms.

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张营,李法云,严霞,李霞,程志辉,沈曼莉,荣湘民.外源K+和水杨酸在缓解融雪剂对油松幼苗生长抑制中的效应与机理.生态学报,2012,32(14):4300~4308

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