There has been an increase in the use of other herbicides for both agricultural and non-agricultural purposes. The use of herbicides in China also has increased faster than that of insecticides and fungicides since last 30 years. The new technique of rice seedling-broadcasting with no-tillage (RSB-NT) is widely used and it was based on the applition of herbicides. The increasing pollution of herbicides in soil and water has been of concerns. The effect of four herbicides including butachlor, bensulfuron-methyl, the mixture of bensulfuron and butachlor, potassium chlorate on typical aquatic animals (Pomacea canaliculata Spix, Tunnel snail) in rice paddy wetland were studied in laboratory. In the immersion experiment, both of the aquatic animals divided into four groups with three concentrations. The results showed that the metabolism of them was affected by these herbicides. The respirations of those two aquatic animals were little affected by potassium chlorate and bensulfuron-methyl, but the respirations of them were significantly inhibited by butachlor and the mixture of bensulfuron and butachlor. Butachlor and the mixture of bensulfuron and butachlor have more infection than potassium chlorate and bensulfuron-methyl to the livability of them. The results also indicated that the NH₄-N, phosphor, and total nitrogen in water with the aquatic animals were affected by these herbicides. Butachlor and the mixture of bensulfuron and butachlor have more influence on excretion rate and phosphor excretion rate of Pomacea canaliculata and Tunnel snail were significantly affected by these four herbicides. In conclusion, the results demonstrated that the best choice for herbicides in rice paddy was the mix of bensulfuron and butachlor, the second was bensulfuron-methyl, the third was butachlor and the last was potassium chlorate according to the impacts of herbicides to the typical aquatic organisms in rice wetland.