Urban land is a social-economic-natural complex ecosystem, with physical, ecological, social and economic attributes. It is the carrier of human socio-economic activities, and the basis to provide the ecological services. Urban land for ecosystem services is the minimum space to guarantee the basic ecosystem services, which play an important role to protect the socio-economic sustainable development of the city and the quality of residents' life. It is not only related with the geographical location, types of natural resources, climate, soil, geology and other natural conditions of the city, but also depended on the urban development level, development orientation and the life quality demands of urban citizens. The construction of urban land for ecosystem services is the basis for urban sustainable development and the natural and artificial ecological unit of the city. Based on the idea of ecological green equivalent, this paper explored the ecological standard to judge if the urban ecological land was reasonable, and established a mathematic model of ecological optimization of land use structure. The land use structure was applied and verified with Ningguo city in Anhui Province as a case. The nature landscape structure of Ningguo is composed of a number of mountains and waters. Based on ecological green equivalent, this article evaluated the current status of natural ecosystems' structure in different space scale. The results showed that green equivalent of region, urban-rural fringe and downtown in Ningguo city were 1.51,1.19 and 0.66, respectively; green plot ratio were 4.89,4.22 and 2.77, respectively, respectively and ecological land per capita were 4898.9,563.3 and 41.7 hm² per capita, respectively. Lacking of green space in the downtown stressed the ecosystem services of urban land. Therefore,urban land for ecosystem services will be planned from different space scales, including the construction of buffer zone in the city and regional area, eco-city construction of main urban areas, the ecological restoration and multifunctional design of the urban green spaces. The suitable human settlement for habitat and fitting socio-economic environment for employment will be created. In order to maintain good urban landscape structure and improved ecosystem services, the natural ecological framework should consist of the key functional areas of water, mountains, urban forests, large-scale urban green space, green wedges, large ecological buffer zones and small ecological corridors. The space network of urban land for ecosystem services of diverse structure and multi-function can be established, including point, line and surface green structure. Through the ecosystem services evaluation, sensitive analysis of the spatial structure, also combining with socio-economic development status, the future urban land use of Ningguo can be divided into two types: ecosystem services area and urban-rural development area. Considering the demand for ecosystem services, the proportion of urban land for ecosystem services should be increased and the Green Line and Blue Line should be strictly controlled as well. This study will provide a scientific method and decision-making basis for strengthening of the ecosystem services of urban areas.