The golden apple snail (Pomacea canaliculata) is considered one of the 100 worst invasive alien species by the Invasive Species Specialist Group of the International Union for Conservation of Nature. The golden apple snail was introduced into China around 1980 from South America for commercial purposes. However, it proved to be a failure on the market over the next few years. Consequently, golden apple snails were disposed of into rivers, canals and paddy fields. Since then, P. canaliculata has resulted in massive rice production losses in South China. Although P. canaliculata is a tropical mollusk species, its cold-hardiness has been reported recently in some studies. Supercooling point is an important index for reflecting the resistance of many creatures including mammals, insects and molluscs in low temperature environments. According to many studies, molluscs also exhibit diverse physiological responses to low temperature environments. However, no studies on supercooling points and physiological responses were performed on P. canaliculata from South China to low temperature stress. In this study, supercooling points of P. canaliculata from South China were determined using a device composed of multimeter determination connected with thermistors. Moreover, mortalities and organ injuries to P. canaliculata of different shell heights during supercooling periods were studied. Results showed that the mean value of supercooling points of snails of different heights was -6.96℃, ranging from -6.21℃ to -7.32℃. The mean value of maximum body temperature in recovery stage was -4.07℃, ranging from -3.07℃ to -4.93℃; the mean supercooling time of P. canaliculata of different heights was 45.97 min, ranging from 18.60 min to 75.34 min. Change in the supercooling point value correlated with the height of P. canaliculata, and the supercooling point of snails with a height of 35 mm≤H<45 mm was significantly higher than those of snails with heights of 5 mm≤H<15 mm, 15 mm≤H<25 mm and 25 mm≤H<35 mm, respectively. It was concluded that the supercooling point of P. canaliculata whose height ranged from 5 to 35 mm was consistent to some extent. The mortality of P. canaliculata in the supercooling stage ranged from 23.33% to 36.67%, and there were no significant differences among mortalities of P. canaliculata of different heights. Mortality of P. canaliculata correlated with increased exposure time, and ranged from 20.9% to 100% in 15 min. This experiment showed that exposure time could greatly influence the mortality of P. canaliculata. Photographs of organs color-stained by 2,3,5-triphenyltetrazolium chloride(TTC) showed a slight red staining on the mantle, which was not obvious on the digestive gland or the kidney of a dead snail, obtained after supercooling for 15 min. Low temperatures resulted in significant organ injury in P. canaliculata. Results obtained from this study will assist further investigation of the ecological adaptation, the northern limit to potential areas of invasion and the survival mechanism of P. canaliculata over winter.