Based on the data collected from 12 research stations in the North-west Daya Bay, South China Sea in spring 2008, the characteristics of macrobenthis community were examined. The results showed that the dominant macrobenthic species were Timoclea scabra, Paphia undulate, Corophium uenoi, Lepidonotus squamatus, Moerella culter and Ophelia acuminate. The macrobenthos community could be divided into four groups with notable differences. Group Ⅰ and Ⅱ were the dominant groups, while the distribution areas of Groups Ⅲ and Ⅳ were relatively small. Overall, molluscs was dominant in quantity in the macrobenthos community but the community was mainly composed of Annelida and Arthropoda, reflecting the characteristics of the community. The dominant macrobenthic species of group Ⅰ were Timoclea scabra, Nephtys oligobranchia, Theora lata, Sigambra hanaokai, Glycinde gurjanvae, Prionospio malmgreni, Timoclea scabra, Turritella bacillum, Corophium tridentium, Phyllodocidae und., Nectoneanthes alatopalpis, Ericthonius pugnax, Pseudopythina sp. and Stylochus ijimai. Species belonging to this group distributed in the nearshore area of Aotou Harbor and Nanhai Petrochemicals Basement Group Ⅱ assemblage includes Paphia undulata, Timoclea scabra, Corophium uenoi, Prionospio malmgreni, Sigambra hanaokai, Ophelia acuminata, Mediomastus sp., Nephtys oligobranchia, Moerella culter, Corophium lamellatum, Paraprionospio pinnata and Prionospio pulchra. These species distributed mainly around the Chunzhou Island where suspended long line culture of oyster was common. The discriminating species for Group Ⅰ were S. hanaokai, N. soligobranchia, P. malmgreni, N. alatopalpis and E. pugnax while group Ⅱ were C. uenoi, P. malmgreni, S. hanaokai, N. oligobranchia, T. scabra, M. sp., Phyllodocidae und., P. pinnata, O. acuminata, C. lamellatum, M. culter and Chaetozone sp. Group Ⅲ assemblage were mainly found in the west side of Mabianzhou, where it was a fishing ground for Arthropoda in Daya Bay. Group Ⅳ was distributed in area where main shipping routes passed through, in the east side of area of Group Ⅱ and in between the area of Group Ⅰ and Ⅲ. For each community group, while group Ⅱ Group Ⅰ was dominated by Annelida and Arthropoda while Group Ⅱ was dominated by arthropoda, annelida and mollusca; such difference is mainly because of the difference in habitat conditions. Diversity of macrobentho community was highest in Group Ⅲ, followed by Group Ⅱ, Group Ⅰ and then Group Ⅳ. Poor habitat condition caused by pollution, sedimentation and other human disturbances were the main reasons for the differences in diversity between groups. In general, macrobenthos community in the North-west Daya Bay of South China Sea was stable in spring. However, community structure of Groups Ⅳ and Ⅲ, which distributed in the main navigation route and fishing grounds, were less stable; suggesting the disturbances from maintenance of navigation channel and fishing activities might have reduced community stability.