The secondary production of macrozoobenthos is an important component of the matter recycle and energy flow in the marine ecosystem. In this field there have been extensive studies abroad, but the domestic research is limited. In order to get a better understanding of the secondary production of macrozoobenthos, seasonal surveys were conducted in the intertidal zone of Jiaozhou Bay. On the basis of the seasonal data in 2009, the secondary production of macrozoobenthos was investigated using three empirical equations: Brey(1990)、Tumbiolo & Downing (1994) and Brey(2001). The results showed that 95 species were found in our study, and there were little variations among seasons. The annual average secondary production varied between 5.3 and 29.9 g AFDW · m^-2 · a^-1, depending on the empirical equations used. The secondary production showed great differences between seasons. The estimation value in summer was much higher than the others,and the values in spring and autumn were similar and were slightly higher than that in winter. The secondary production of macrozoobenthos in low-tidal area E was the highest, and was slightly lower in middle-tidal area B&C, much lower in middle-tidal area D and the lowest in high-tidal area A. However, there were slight variations in the secondary production among different seasons and among the methods used. The secondary production was the highest in the tidal area D in spring, while the highest value was in the tidal area E in summer and autumn, and B in winter; the secondary production was relatively low in the tidal area A&C in all the seasons, and the tidal area D had also low value in summer. The aquaculture of bivalves may contribute mostly to the changes of spatio-temporal patterns of the macrozoobenthic secondary production in the study area. The three empirical equations showed some differences in the estimations of the secondary production. In spring, the estimations from the three models were significantly different; and in summer the estimations from Brey(1990)and Brey(2001) equations were similar, while the values of Tumbiolo & Downing (1994) model were low. In autumn and winter, the estimations of Tumbiolo & Downing (1994) and Brey(2001)equations were close to each other, and Brey(1990)method deviated from them significantly. The Wilcoxon test showed that estimations of secondary production from the three methods differed significantly, which presented a caveat for the applications of the empirical equations. According to our results, it inferred that Brey(1990)and Brey(2001)models were suitable for the estimation of the secondary production of the entire year, and the Tumbiolo & Downing (1994) was suitable for the estimation in autumn and winter. Brey(1990)and Tumbiolo & Downing (1994) models were convenient methods for the estimation of the secondary production of macrozoobenthos, but they simplified the environment factors and the compositions of the benthos community, which might impact the accuracy of the estimations. The species composition of the macrozoobenthic community and the water temperature of the study area may be the main reasons for the different estimated values from the above equations in our study.