The objective of this study was to investigate the effects of atmospheric elevated O3 on concentrations of DTPA-extractable microelements of paddy soil in the rice season of wheat-rice rotation. Ozone Free-air Concentration Enrichment (O3-FACE) system at Xiaoji town, Jiangdu County, Jiangsu Province (32°35′5″N, 119°42′0″E) was used in this study. Of six plots in O3-FACE system, three plots were under elevated O3 concentration \[O3\] (FACE) and the other three were under ambient \[O3\] (ambient). The target \[O3\] for FACE plots was 50% higher than the ambient \[O3\]. Each plot had an area of 240 m2. Any one of the plots was separated from the other plots by at least 70 m to avoid cross-contamination. Concentrations of DTPA- extractable Fe, Mn, Cu and Zn at different soil depths (0-5 cm, 5-10 cm and 10-15 cm) were determined under ambient and elevated ozone concentration treatment at different stages of the rice season in 2009. The results showed that elevated O3 increased the concentrations of DTPA- extractable Fe, Mn and Zn in soil at 0-15 cm depth with 10.0%, 8.1% and 40.3%, respectively and significantly increased the concentrations of DTPA-extractable Cu with 5.4% (P=0.049). Vertical distribution of DTPA-extractable microelements in soil differed with different microelements. Concentrations of DTPA-extractable Fe and Zn in soil decreased with soil depth, while concentration of DTPA-extractable Mn in soil increased with soil depth and concentration of DTPA-extractable Cu in soil was relatively stable at different depths. There had diverse effects of O3 enrichment on concentration of soil DTPA-extractable microelements at different depth of soil. The effect of O3 enrichment on concentration of soil DTPA-extractable Fe increased with soil depth, while the effects of O3 enrichment on concentration of soil DTPA-extractable Mn, Cu and Zn decreased with soil depth. Atmospheric elevated O3 significantly increased concentrations of DTPA-extractable Cu at 0-5 cm, 5-10 cm and DTPA-extractable Mn at 0-5 cm depth of soil with 13.2% (P=0.013), 8.9% (P=0.026) and 30.2% (P=0.003), respectively. The possible mechanisms related the effects of elevated O3 on concentrations of DTPA-extractable microelements of paddy soil were discussed in this paper. Results from this study indicated that the geochemistry cycle of microelements in paddy soils in the agricultural ecosystem can be influenced by elevated O3 in the atmosphere; in order to understand the impact mechanism of elevated O3 on concentrations of DTPA-extractable microelements in soil, soil properties and rice growth, together with the microelement status at different soil depths should be considered. Based on the previous studies in CO2-FACE system, CO2 enrichment also can affect concentration of soil DTPA-extractable microelements, therefore, the combined influence of simultaneously elevated O3 and CO2 on the geochemistry cycle of microelements in paddy soils should be studied in the future.