Arbuscular mycorrhizal fungi (AMF) are important functional components of ecosystems and are the most widely distributed microbes among the soil microorganisms. Glomalin, which is produced by AMF, is a kind of glycoprotein that can be extracted from soil. Its ubiquity, coupled with apparent recalcitrance of glomalin and consequent long residence time in soil, can result in glomalin comprising large pools of soil carbon and nitrogen. Hippophae rhamnoides L is one of the excellent sand-fixation plants that can not only well adapt to arid desert environment, but also fix sand and improve the texture or fertility of soil, so as to keep the balance and stability of the desert ecosystem. In this paper, the spatial distribution of AMF and glomalin, and the relativity of AMF and glomalin with environmental factors in rhizosphere of Hippophae rhamnoides L were investigated in farming-pastoral zone between Inner Mongolia and Hebei province. In July 2009, we collected the root and soil samples from three different sample sites, which are Da Liandi, Huang Liutiao and east of Duo Lun, respectively. Root samples were collected from four robust Hippophae rhamnoides L, and soil samples were collected from three sample sites and divided into five layers on the basis of depth, from top to bottom: 0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm, 40-50 cm, respectively. The results showed that a good symbioses relation is formed between AMF and Hippophae rhamnoides L. Mycorrhizal type is Arum-type. The distribution and activity of AMF, the content of total extractable glomalin (TEG) and easily extractable glomain (EEG) were significantly affected by ecological conditions and soil depth. In Daliangdi and Duolun sites, there was no obvious difference in the spore density, but in Huangliutiao site was much higher; the colonization of AMF showed the same trend with spore density. Meanwhile the arbuscule was only found in Daliangdi site. The maximal value of the spore density occurred at the 0-10 cm soil layer in three sites, and then decreased with the increasing of the soil depth, and the percentage of AM fungal colonization showed the same trend in Daliangdi site, while no obvious difference was found in the variation laws of different structures of AM fungal colonization in other sample sites. The highest colonization rate and spore density of AMF were found in the 0-30 cm soil layer in each site. The highest contents of TEG and EEG occurred at the 0-10 cm soil layer, and then gradually decreased with the increasing of soil depth. Spore density had significantly positive effects on total, hyphal and vesicular colonization. Different structures of AMF had significantly positive effects on soil pH, organic C, alkaline N, available P , urease and alkaline phosphatase. Arbuscular colonization had a positive effect on alkaline N and urease. The result of multiple linear regression equation showed that different colonial structures of AMF and different soil factors had different impact on the content of TEG and EEG. For TEG: hyphae>spore> vesicale; organic C> available P> acid phosphatase. For EEG: vesicale >spore> hyphae; organic C> available P. Spore density, colonisation of hyphae, the contents of TEG and EEG were remarkably correlated with the contents of soil nutritions and the activities of soil enzymes, and in the future research, these indexes can be used as parameters to monitor the development of AMFcommunity, organic carbon dynamic and nutrition cycle in sand soil.