In terrestrial soils, carbon (C) and nitrogen (N) fluxes are primarily controlled by small but highly bio-reactive labile pools, while long term C and N storage is determined by the long-lived recalcitrant fraction. Changes in the size of labile, recalcitrant, and microbial carbon and nitrogen pools as well as redistribution among them may considerably affect terrestrial soil C and N storage and turnover rates. Hence, analyzing the labile and recalcitrant fraction of soils may improve our ability to detect and predict changes in soil C and N dynamics.This study used OTCs (Open Top Chambers) and clipping plus dung application and sulfuric acid hydrolysis to quantify changes in the labile and recalcitrant C and N fractions in soils from alpine meadows in the eastern Qinghai-Tibetan Plateau.Further, the effects of warming and grazing on labile and recalcitrant C and N fractions and microbial biomass in these soils were investigated. Using OTCs to warm soils resulted in an increase of 2.25℃ in daily mean air temperature at 30 cm above the ground and an increase of 1.17℃ in soil temperature at 10 cm depth, yielding significantly decreased labile C and N pools along with diminished recalcitrant C pool in the short-term, indicating an increase in soil respiration. Grazing markedly increased labile C and N pools I due to dung application, resulting in increments of 567 mg C kg-1 dry soil (26.13%) and 28mg N kg-1 (11.21%). The effects of warming and grazing on labile and recalcitrant pools counteracted each other, which led to non-significant changes in the combined treatments. Although warming or grazing alone did not alter the microbial biomass C pool, the combined treatments greatly increased it, by an increment of 147 mg C kg-1 dry soil (41.91%). The interaction between grazing and sampling dates was significant and the effects of grazing decreased with time. For all measured pools, soil microbial biomass C and N showed significant interactions between warming, grazing, and sampling dates, indicating that microbial biomass C and N in these soils are very sensitive to environmental change. Our results suggest that climatic warming slightly affects organic C and N fractions in meadows where grazing occurs and the effects of nutrients returned to the ecosystem through dung deposition cannot be ignored. In the eastern Qinghai-Tibet Plateau, alpine meadow ecosystems are distributed widely. Climate warming and disturbances such as grazing are main anthropogenic factors that drive changes in the structure and function of these ecosystems. According to our research, the effects of warming and grazing on labile and recalcitrant C and N pools counteracted each other, thus warming and grazing might only have slightly effects on alpine meadow soil C and N storage in the short-term.