Formulation of nature reserves groups is one of important approach for optimizing the spatial patterns of nature reserves, and improving the effect of nature reserves network. In this study, based on habitat evaluation and connectivity analysis, the approach and method for optimizing spatial patterns and function zoning of reserves was analyzed using the case study of giant pandas (Ailuropoda melanoleuca) reserves in Qinling mountains range. Results showed that, 17 panda nature reserves were established or in establishment, which almost formed a reserves group and covered 50% of panda habitat. But 3 reserves in the east were isolated from the others, and core zones of the 17 reserves were isolated into 20 parts. This pattern will reduce the effect of reserves systems in protecting pandas and other endangered species. For optimizing the spatial patterns of the nature reserves, new reserves including Xushui River and Jingjiliang were proposed, and the number of core zones was suggested to 4 parts according to the spatial distribution of panda population. Besides, three linkage areas were proposed for facilitating the pandas exchange and movement among different populations. The study is expected to provide a scientific basis for nature reserves construction in this mountain range, and to benefit the establishment of nature reserves group in other places and the optimization of entire nature reserves systems in China.