In order to improve grain yield of bread wheat (Triticum aestivum L.), the bleeding intensity of neck-panicle node and spike traits changes in wheat were investigated after spraying ABA (12mg/kg, 45 mL/m²).In this study, two currently high-yield wheat cultivars Shannong 8355(SN8355) with the large spike and Shannong 15 (SN15) with the multiple spike were used to identify their effects on bleeding intensity in neck-panicle node,spike traits and grain yields after spraying ABA during the filling stage. ABA solution was sprayed at the late booting stage (T1) and 3 days after anthesis (T2), respectively. The results indicated that bleeding intensity in neck-panicle node was significantly greater at the early filling stage but weaker at the late filling stage. Further analysis indicated that the bleeding intensity showed a single peak at the 14th day after anthesis in the multiple spike wheat cv. SN15 but two peaks at 14th and 28th days after anthesis in the large spike wheat cv. SN8355, respectively. After spraying ABA, 5.20% and 3.93% bleeding intensities in neck-panicle node were increased for Shannong 15 and Shannong 8355 at the late booting stage, respectively, while 4.26% and 5.89% at 3rd day after anthesis. Similarly, 6.98% and 3.31% group bleeding intensities were increased for Shannong 15 and Shannong 8355 at the late booting stage, respectively, while 3.32% and 2.96% were increased at 3rd day after anthesis. The changing tendency of grain kernel weight and volume at each grain position was generally T2＞CK＞T1. Grain number in different position showed more significant influence on 3rd and 4th grain position than 1st and 2nd grain position after spraying ABA, especially for increasing the ratio of the 4th position of the large spike cultivar Shannong 8355 from 2.05% to 7.18%. Spraying ABA at different stages decreased the number of sterile spikes (0.2-0.7) to some extent, but increased the grain number per spike with highest increment of 31.31% and average seed setting rate by 2.79% for two cultivars surveyed. Both of them showed the tendency of T1＞T2＞CK. The results also indicated that grain weight increased by 7.90%-19.01% and finally grain yield increased by 4.08%-9.81% for two cultivars, and both of them showed the tendency of T2＞T1＞CK. Statistical analysis indicated that bleeding intensity of neck-panicle node was closely associated with grain weight during almost all growing stage and the similar relationship was also observed between group bleeding intensity and grain yield. Therefore, it is very useful for wheat improvement of grain yield to examine the significant positive correlation of group bleeding intensity and grain yield at 7th day after anthesis. This study suggested that the optimum ABA sprayed at appropriate growth stage possibly could improve bleeding intensity, optimize spike development and increase grain yield of wheat.