Measurements of the global distribution of tropospheric NO₂ column densities have become available with the Ozone Monitoring Instrument (OMI) which is launched in September 2004 on board the Aura satellite. The spatial and temporal characters of NO₂ over Zhejiang Province is analyzed using monthly averaged tropospheric NO₂ column densities from OMI measurement from January 2005 to December 2009. In addition, with the NO₂ (2005 2009) column densities data and the land-use map of Zhejiang province, the characters of NO₂ concentration over different ecosystem have been analyzed in Zhejiang Province. The results indicated that the tropospheric NO₂ column densities in Zhejiang increased distinctly from 2005 to 2009, and it showed distinct regional and seasonal variation characteristics. It has the highest concentration in winter while lowest in summer. It also presents zonal distribution and decreasing from northern to southern. Under the influence of the urbanization process, the highest NO₂ column densities appeared at cities and metropolis which are located in the Hangzhou-Jiaxing-Huzhou plain, northern area of Ningbo as well as the west and east of Zhejiang. Moreover the lowest column densities appeared at the forest areas which are in the south and west of Zhejiang. The Tropospheric NO₂ column densities have been significantly distinction over different ecosystems. In addition, it has the higher NO₂ column density over urban, cropland and water while lower values over forest. There are different performance characteristics in five land use types along with the NO₂ column densities increasing year by year. The serious pollution of NO₂-Ⅰtype increased significantly over the troposphere of cropland, water bodies and urban land, while the performance of less contaminated NO₂-Ⅲ type increased significantly over the troposphere of forest and grass land.