夜间增温和施肥对川西亚高山针叶林两种树苗根际效应的影响
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国家自然科学基金资助项目(30800165); 中国科学院知识创新工程重要方向项目(KSCX2-YW-Z1023); 中国科学院"西部之光"人才计划项目;中国科学院成都生物研究所领域前沿资助项目(Y0B2021100)


Responses on rhizosphere effect of two subalpine coniferous species to night-time warming and nitrogen fertilization in western Sichuan, China
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    摘要:

    开展植物根际效应对全球变化响应的研究是深入认识根际微生态系统中植物根系与土壤微生物相互作用过程及机制的关键。以川西亚高山针叶林两种主要树种幼苗--云杉(Picea asperata)和岷江冷杉(Abies faxoniana)为研究材料,采用红外辐射模拟增温和外施NH4NO3氮肥的方法,研究了夜间增温和施肥对两种幼苗根际效应的影响。结果表明:红外辐射增温导致气温增加了1.62 ℃,土壤5 cm和10 cm层月均温度显著增加了2.89 ℃和3.10℃。增温和施肥处理对两种幼苗不同参数根际效应的影响各不相同,表现为不同程度的正根际效应、负根际效应或者无影响。增温使云杉幼苗根际与非根际土SMB-C含量均显著增加(分别为42.28%和31.02%),非根际土有机碳含量降低了7.03%;而增温对两种幼苗土壤肥力因素根际效应的影响总体并不显著,增温仅对云杉全氮有显著的负根际效应(79.43%),而岷江冷杉通过根际土全氮和SMB-N含量的增加,其根际效应大小在增温处理下显著增强。施肥处理和两因子的联合作用显著提高了两种幼苗的NO-3-N、NH+4-N和云杉非根际土SMB-N含量,并使岷江冷杉NH+4-N表现出正根际效应,而云杉SMB-N表现出明显的负根际效应(120.80%和253.06%)。这种响应差异可能与不同植物种类地下碳分配及其植物根系所吸收的养分有关,从而赋予了不同植物种类在未来全球变化背景下可能具有不同的适应力和竞争优势,并进一步对亚高山针叶林地下过程及其早期更新产生潜在影响。

    Abstract:

    As a component in the belowground ecosystem, rhizosphere microecological system may affect global carbon cycling process. The recent studies suggest that rhizosphere processes may play an important role in mediating ecosystem function such as net primary production, decomposition, and C storage to climate change. However, the mechanisms by which roots affect rhizosphere processes are still poorly understood, and the responses on rhizosphere effects to exogenous warming and nitrogen addition are little known. To obtain a clear knowledge of rhizosphere responses of the different species to warming and nitrogen addition is very important to understand the mechanisms in belowground ecosystem to global climate change. Picea asperata and Abies faxoniana are two dominant species of subalpine coniferous forest in western Sichuan, China. A controlled experiment was carried out to investigate the responses on rhizosphere effect of P. asperata and A. faxoniana seedlings and the differences between two species to night-time warming (ambient temperature; infrared heater warming) and nitrogen fertilization (0; 25g m-2 a-1 N). The responses on their rhizosphere effects to simulated global change play an important role to survey the interaction between plant-root and soil-microbe, which can provide insights into rhizosphere micro-ecological system, manage and restore the vegetation of subalpine coniferous forest. The results were shown as follows: (1) Contrast to the control, mean air temperature was 1.62 ℃ higher in the warming plot than the control plot, meanwhile soil temperature at 0 cm, 5 cm, 10 cm and 15 cm depths also increased by 1.97 ℃、2.89 ℃、3.1 ℃ and 2.37 ℃ respectively; In addition, warming and ambient air relative humidity are 79.24% and 87.99%. (2) Different responses (positive rhizosphere effects, negative rhizosphere effects or no rhizosphere effects) were found in soil properties for both species under warming and nitrogen fertilization. Warming generally resulted in increased by 42.28% and 31.02% on microbial biomass C (SMB-C) of rhizosphere and bulk soil for P. asperata respectively. While soil organic C of the bulk soil was significantly decreased by 7.03% by warming. In general, warming did not affect the rhizosphere effects of soil properties in both tree seedlings. However warming showed significantly negative rhizosphere effects on total N for P. asperata seedling, which significantly reduced the rhizosphere effects by 79.43%; Rhizosphere effects of A. faxoniana was significantly increased mainly due to the increased total N and microbial biomass N (SMB-N) in the rhizosphere. NO-3-N and NH+4-N of two tree seedlings were significantly increased by nitrogen fertilization and interaction between warming and nitrogen fertilization as well as microbial biomass N (SMB-N) of the bulk soil for P. asperata. Meanwhile, an remarkably negative effect on SMB-N was observed for P. asperata. In addition, interaction between warming and nitrogen fertilization showed significantly positive effects on NH+4-N for A. faxoniana. Different competitive and adaptive strategies between the two conifers may explain their different responses of rhizosphere effects to stimulated warming and nitrogen fertilization, which may be important to give insights into potential influences on belowground ecological processes and early regeneration dynamics for subalpine coniferous forest ecosystems in face to future climate change.

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卫云燕,尹华军,刘庆,黎云祥.夜间增温和施肥对川西亚高山针叶林两种树苗根际效应的影响.生态学报,2011,31(3):698~708

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