In order to investigate the effects of sewage sludge amendment on physico-chemical properties of mine tailings and physiological responses of Cinnamomum camphora to the mixture, three different sites of sewage sludge (Huangyan (HY), Linhai (LH) and Luqiao (LQ)) were collected, and then mixed with mine tailings in which the mass percentage of sewage sludge was 0%, 25%, 50% and 75%, respectively. Results showed that sewage sludge amendment increased the nutrient of mine tailings and changed the heavy metal composition of mine tailings. With the increase of sludge amendment ratios, organic matter, total N and total P were significantly increased, while pH and cation exchange capacity were significantly decreased; total and DTPA-extractable contents of Cu, Cd and Zn except for total Zn were significantly increased, while total and DTPA-extractable contents of Pb were significantly decreased. It can be inferred that the composition of heavy metals in the mixture was related with the original heavy metal characteristics of both mine tailings and sewage sludge. The biomass of leaves, stems and roots, and chlorophyll contents consistently decreased with the increase of LQ sewage sludge amendment ratios, which may be related with the high Cu contents in LQ sludge. The biomass of leaves and stems, and chlorophyll contents of C.camphora grown at 25% and 50% sewage sludge in HY and LH were more than those at control (0), whereas they were significantly decreased at 75% sewage sludge content. The change of MDA contents, the indicator of biotoxicity, was reverse to those of biomass and chlorophyll. Therefore, the 50% amendment ratios of HY and LH sludge was assumed the best remediate measures due to the least biotoxicity to C.camphora. The biotoxicity had a closely relation with the accumulation of heavy metals in C.camphora. Cu, Cd, Pb and Zn contents of leaves and roots at 25% and 50% ratios of HY and LH sewage sludge were more than those of control (0%), but Cu, Cd and Zn contents of leaves and roots greatly increased at 75% ratios of HY and LH sewage sludges. However, with the increase of LQ sewage sludge amendment ratios, Cu, Cd and Zn contents of leaves and roots consistently increased, and were more than those of HY and LH sludge treatments. Notably, Pb in the leaves and roots consistently decreased with the increase of LQ sewage sludge amendment ratios, which may be related to the decrease of total and DTPA-extractable contents of Pb. Only in LQ sludge treatments, the Cu, Cd and Zn contents of leaves and roots had positive correlation with their DTPA-extractable contents, respectively, which may be related to different organic matter composition between LQ and other two sludge types. Therefore, heavy metal contents and organic matter composition in the sewage sludge could help to predict the bio-toxicity to plants when mine tailings were amended with sludge.