In this paper, advantages of MODIS-PSN, CASA, GLO-PEM, VPM model based on light use efficiency (LUE) are used for reference, and typical characteristics of vegetation LUE and environment in the study area are taking into account. Based on relative literature and measured NPP, the maximum LUE values of main vegetation in the study area are simulated. The maximum LUE values of grassland and shrubland are calculated through three steps in order to reduce calculating errors. Based on Beer′s law, environmental stress factors of LUE is calculated by evaporative fraction algorithm and TEM model. The LUE values of main vegetation in Qinghai province are simulated, and their spatial distributions and dynamic changes are analyzed. The result indicates that the mean LUE value ranges from 0.026gC/MJ to 0.403gC/MJ in 2006 in Qinghai province, and the average is 0.096gC/MJ. The LUE of Vegetation with obvious zone distributions gradually increases from north-west to south-east. The LUE value in Qinghai province largely varies with the season. The monthly mean LUE of vegetation changes from 0.057gC/MJ to 0.157gC/MJ in 2006, and the highest appears in July. The main accumulative period of LUE is from May to September.