High soil indigenous N supply of paddy soil is one of the major reasons of low fertilizer-N use efficiency. Reducing amount of N fertilizer annually can significantly improve fertilizer-N use efficiency and abate environmental stresses. In this study, by analyzing the data of soil total nitrogen and rice yield of long-term field experiment(from 1990 to 2006) in reddish paddy soil, the impact of different fertilizer regimes on soil N storage, N agronomic use efficiency(AUE) and the feasibility of reducing the amount of N fertilizer application by 33.3% were studied. Results showed that long-term cycling of organic matter(17 a) significantly increased soil total N storage in upper layer by 18.8%，while no obvious changes were detected from the plots only applying chemical fertilizers.In the treatment of chemical fertilizer reduction, the accumulation of soil nitrogen in plough layer was higher than that in NP and NPK treatments. Compared with NPK，chemical fertilizer reduction regime resulted in relative error of Grain yield of 3.2% and the increase of input-N AUE 12.0%. Therefore, the economical N fertilizer application under the recycling of half rice straw incorporation was 180 kg?hm-2 while rice yield could maintain about 10 t?hm-2.