Field trials were carried out to investigate effects of four N application rates (0, 56.25, 112.5 and 168.75 kg?hm-2) on rhizosphere microflora and fusarium wilt occurrence of faba bean in wheat and faba bean intercropping. Results showed that rhizosphere microbe population of faba bean were increased significantly with increasing N application,in both intercropping and monocropping, the peak value of microbial population appeared in N2 (112.5 kg?hm-2) treatment. No significant effects of N application on soil microbe diversity were detected, but N application reduced intercropped faba bean fusarium wilt occurrence, the lowest disease incidence and index was appeared with N2 (112.5 kg?hm-2) treatment. In comparison with monocropping, the amount of rhizosphere bacteria, fungi, actinomyces, total microbes and microbe diversity of intercropped faba bean significantly increased, the promoting effects of rhizosphere microflora with N application, especially with N0, N1(56.25 kg?hm-2)and N2 (112.5 kg?hm-2) was also prominent, the amount of fungi and actinomyces population increased significantly. Fusarium wilt incidence and index of intercropped faba bean significantly decreased with N0, N1 and N2 application rates. Rational nitrogen application could effectively regulate rhizosphere microbial population of faba bean, and it could be also an effective measure to control faba bean fusarium wilt occurrence in wheat and faba bean intercropping.