The effect of ship noise on the secretion of cortisol was studied using the perch, Lateolabrax japonicus, and the yellowfish, Pseudosciaena crocea in controlled laboratory conditions. The results showed that after ship noise exposures (155dB,1μPa2/Hz), cortisol level in both fish either increased, then gradually decreased to the original levels, or increased and remained at high levels. Blood cortisol levels in the perch reached 112.23ng/mL after 60min exposure. Yellowfish cortisol levels reached 17.18ng/mL after 20-60 min exposure. Perch cortisol levels (CL155,CL138) relative to the time (T) of intermittent noise exposure can be expressed as CL155=33.507e0.2425T(r=0.8939,P<0.01); CL138=36187e0.1862T(r=0.8002, P<0.01), Yellowfish cortisol levels (CH155, CH138) in the same conditions may be expressed as CH155=3.1208e0.2535T (r=0.8833,P<0.01); CH138=2.8369e0.1706T (r=0.8064,P<0.01）. Perch (CL) and yellowfish (CH) cortisol levels relative to ship noise (S) can be expressed by the model: CL=33.05154e0.2461S, r=0.9563;CH=39706e0.2401S,r=0.9114.