Urban wetland is an important part of urban landscape. Its ecological function plays a significant role in the development of urban eco-environment. But, with a rapid expansion of urban land and citizenry on the eastern suburb of Nanjing, most of wetlands lost. As results, landscape surrounding each remnant wetland has been changed. In this study, based on remote sensing and GIS techniques, the landscape heterogeneities were identified at sub-watershed scale for 17 wetlands in the eastern suburban of Nanjing. Farther more, these 17 sub-watersheds were divided into three classes that can best explain land use characteristics by clustering analyses of SPSS. The collection of samples for water quality analysis was performed in March, 2008 to March 2009， and samples were collected in 17 wetlands once a month. Our research results found that: ① land use of urban wetland impacted water quality prominently. Higher percent forest cover was associated with better water quality.② the index of ZQI (Increased patch), XJI (Decreased Patch), R_ZXI (contrast of ZQI and XJI) was related to higher water quality. R_ZXI increase were associated with decrease of Total nitrogen (TN) and Chemical Oxygen Demand (COD) when R_ZXI<-1; and R_ZXI increase were associated with increase of TN and COD when R_ZXI>-1. However, the total phosphorus (TP) change exhibited opposite to TN. ③ Evenness of landscape for wetlands were also related to water quality. Higher evenness were associated with lower TN, CODMn, and it’s opposite to TP.