This paper investigated the effect of ginsenosides （four ginsenoside mixtures including total ginsenosides, panaxadiol ginsenosides, panaxatriol ginsenosides, ginsenosides\|Rb group, and two monomer ginsenosides\| ginsenoside\|Rb3 and ginsenoside\|Re） on the germinating of ginseng seeds, the root length of seedlings, the fresh weights, the activity of antioxidant enzyme in radicles and the content of MDA in vitro. Results have shown that: the above ginsenosides all had inhibitory effects on seed germinating, the growth of seedling roots and the fresh weight, with the inhibitory effect increasing with treatment concentration. The SOD, POD and CAT activity in ginseng roots all increased when treated by total ginsenosides, panaxadiol ginsenosides and panaxatriol ginsenosides at various concentrations, indicating that the activities of enzymes increase with concentration. When treated by ginsenoside\|Rb group, SOD activity showed no significant difference over the control group at low to medium treatment concentrations, and was lower than the control group at medium to high treatment concentrations; POD activity significantly increased at medium to high treatment concentration, and dropped to a level lower than the control group when treated by high treatment concentrations; CAT activities were all lower than the control group; when treated by ginsenoside\|Rb3, SOD activities were all lower than the control group, POD activity equaled to the control group at lower treatment concentrations, and was significantly lower than the control group at medium to high treatment concentration; CAT activity gradually decreased, and was slightly higher than the control group at low to medium treatment concentrations, and was lower than the control group at high concentrations; when treated by ginsenoside\|Re, SOD and POD activities were all significantly lower than the control group. All ginsenosides treatments were accompanied by an increased MDA content.