Using TCT-GC/MS technique, the comparative analysis of composition and relative percentage of volatile organic compounds in leaves of Eupatorium adenophorum infested with and without cotton aphids under control conditions at different temperatures and lights were performed. The Results showed that, composition of volatile compounds released from both the control and infested leaves were not affected, but the relative percentage of those were changed under the different temperatures and lights. When the light intensity reached 300-400μmol?m-2?s-1, the relative percentage of most monoterpenes were more abundant in infested leaves than in control leaves, while the relative percentage of sequiterpene decreased significantly. Furthmore, the relative contents of green leaf volatiles, such as Hexanal, 2-Hexenal and most monoterpenes, in the infested leaves of E. adenophorum significantly increased as temperature went up between 15℃ and 30℃, but sequiterpene decreased. These data may suggest that suitable temperature and light affected the chemical fingerprint of E.adenophorum, and hence influencing the aphids feeding.