The dynamics of CO2 concentration in Moso bamboo(Phyllostachys edulis) plantation was studied with CID-301 portable photosynthesis system in Huitong County, Hunan Province. The results showed that the soil total respiration rate, heterotrophic respiration rate, autotrophic respiration rate and litter annual respiration rate of Moso bamboo plantation were 2.13, 1.44, 0.69 and 0.31 μmolCO2?m-2?s-1, respectively. It was obvious that soil respiration rate exhibited strongly annual and daily dynamic pattern in Moso bamboo plantation. The annual dynamic pattern was represented with a single-peak curve. The respiration rates increased with increasing atmospheric and soil temperature from January to July. Total soil total respiration rate, heterotrophic respiration rate, autotrophic respiration rate and litter respiration rate reached their highest values in August, being 4.95, 3.01, 1.94 and 0.80 molCO2?m-2?s-1, respectively. All of these respiration rates discreased gradually with falling temperature from August to January or February in the next year. At this time, the respiration rates reached their lowest values, which were 0.76, 0.70, 0.06 and 0.05 μmolCO2?m-2?s-1, respectively. The daily variation of total soil respiration rate, heterotrophic respiration rate, autotrophic respiration rate and litter respiration rate of Moso bamboo plantation could also be represented with a single-peak curve. They usually showed a strong consistency with temperature. From 6:00 to 14:00, the respiration rates increased with increasing temperature, and reached the highest value from 16:00 to 18:00. After this time, they decreased until the time from 4:00 to 8:00 in next day. The amount of CO2 released from Moso bamboo plantation was 33.94tCO2?hm-2?a-1, including 59.5% heterotrophic respiration, 28.3% autotrophic respiration and 12.2% litter respiration.