To investigate the correlation between urbanization and butterfly diversity, four different habitats, i.e. forest, farmland, city and wetland, in Guangzhou were surveyed between 2005 and 2006. 73 species of butterflies from 46 genera in 10 families were recorded. Margalef species richness and Shannon-Wiener indices were used to analyze the species and community diversity of butterfly in the four urbanized regions. The results showed that both indices were the highest in forest regions and the lowest in wetland regions. While, wetland region showed the highest evenness index, followed by farmland, forest and city regions; also, wetland regions have the highest dominance index, followed by city, farmland and forest regions. The highest similarity, in term of numbers of common species, occurred between forest and farmland; in contrast, wetland regions had the most distinct species composition as compared to other regions.